Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction

Lei Shi, Shin-ichi Miyoshi, Kewei Bi, Masami Nakamura, Mieko Hiura, Kenichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A total of 120 strains of Vibrio mimicus, 51 clinical and 69 environmental, were examined for the presence of three types of hemolysin genes (vmh, tdh and hlx) by PCR. Ninety-six percent (115) of the strains contained at least one of these hemolysin genes. Only 5 strains from the environment were missing all three hemolysin genes. The tdh was only found in 20 of the 51 clinical isolates of V. mimicus. This may indicate that the tdh gene is a virulence determinant of V. mimicus. More than 90% of the strains isolated from both the environment and patients possessed the vmh gene. Two clinical isolates possessed the hlx gene alone and had no other enterotoxic factors.

Original languageEnglish
Pages (from-to)63-65
Number of pages3
JournalJournal of Health Science
Volume46
Issue number1
Publication statusPublished - 2000

Fingerprint

Vibrio mimicus
Hemolysin Proteins
Polymerase chain reaction
Genes
Polymerase Chain Reaction
Virulence

Keywords

  • Hemolysin gene
  • PCR
  • Vibrio mimicus

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction. / Shi, Lei; Miyoshi, Shin-ichi; Bi, Kewei; Nakamura, Masami; Hiura, Mieko; Tomochika, Kenichi; Shinoda, Sumio.

In: Journal of Health Science, Vol. 46, No. 1, 2000, p. 63-65.

Research output: Contribution to journalArticle

Shi, L, Miyoshi, S, Bi, K, Nakamura, M, Hiura, M, Tomochika, K & Shinoda, S 2000, 'Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction', Journal of Health Science, vol. 46, no. 1, pp. 63-65.
Shi, Lei ; Miyoshi, Shin-ichi ; Bi, Kewei ; Nakamura, Masami ; Hiura, Mieko ; Tomochika, Kenichi ; Shinoda, Sumio. / Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction. In: Journal of Health Science. 2000 ; Vol. 46, No. 1. pp. 63-65.
@article{87a36c41ec00427aa5c205dc6cd59cb8,
title = "Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction",
abstract = "A total of 120 strains of Vibrio mimicus, 51 clinical and 69 environmental, were examined for the presence of three types of hemolysin genes (vmh, tdh and hlx) by PCR. Ninety-six percent (115) of the strains contained at least one of these hemolysin genes. Only 5 strains from the environment were missing all three hemolysin genes. The tdh was only found in 20 of the 51 clinical isolates of V. mimicus. This may indicate that the tdh gene is a virulence determinant of V. mimicus. More than 90{\%} of the strains isolated from both the environment and patients possessed the vmh gene. Two clinical isolates possessed the hlx gene alone and had no other enterotoxic factors.",
keywords = "Hemolysin gene, PCR, Vibrio mimicus",
author = "Lei Shi and Shin-ichi Miyoshi and Kewei Bi and Masami Nakamura and Mieko Hiura and Kenichi Tomochika and Sumio Shinoda",
year = "2000",
language = "English",
volume = "46",
pages = "63--65",
journal = "Journal of Health Science",
issn = "1344-9702",
publisher = "Pharmaceutical Society of Japan",
number = "1",

}

TY - JOUR

T1 - Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction

AU - Shi, Lei

AU - Miyoshi, Shin-ichi

AU - Bi, Kewei

AU - Nakamura, Masami

AU - Hiura, Mieko

AU - Tomochika, Kenichi

AU - Shinoda, Sumio

PY - 2000

Y1 - 2000

N2 - A total of 120 strains of Vibrio mimicus, 51 clinical and 69 environmental, were examined for the presence of three types of hemolysin genes (vmh, tdh and hlx) by PCR. Ninety-six percent (115) of the strains contained at least one of these hemolysin genes. Only 5 strains from the environment were missing all three hemolysin genes. The tdh was only found in 20 of the 51 clinical isolates of V. mimicus. This may indicate that the tdh gene is a virulence determinant of V. mimicus. More than 90% of the strains isolated from both the environment and patients possessed the vmh gene. Two clinical isolates possessed the hlx gene alone and had no other enterotoxic factors.

AB - A total of 120 strains of Vibrio mimicus, 51 clinical and 69 environmental, were examined for the presence of three types of hemolysin genes (vmh, tdh and hlx) by PCR. Ninety-six percent (115) of the strains contained at least one of these hemolysin genes. Only 5 strains from the environment were missing all three hemolysin genes. The tdh was only found in 20 of the 51 clinical isolates of V. mimicus. This may indicate that the tdh gene is a virulence determinant of V. mimicus. More than 90% of the strains isolated from both the environment and patients possessed the vmh gene. Two clinical isolates possessed the hlx gene alone and had no other enterotoxic factors.

KW - Hemolysin gene

KW - PCR

KW - Vibrio mimicus

UR - http://www.scopus.com/inward/record.url?scp=0034049549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034049549&partnerID=8YFLogxK

M3 - Article

VL - 46

SP - 63

EP - 65

JO - Journal of Health Science

JF - Journal of Health Science

SN - 1344-9702

IS - 1

ER -