Presence of defensin in epithelial Langerhans cells adjacent to oral carcinomas and precancerous lesions

Nobuyoshi Mizukawa, Katsumi Sugiyama, Eiki Yamachika, Takaaki Ueno, Katsuaki Mishima, Shin Takagi, Toshio Sugahara

Research output: Contribution to journalArticle

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Abstract

We aimed to immunohistochemically study the localization of defensin (HNP(s)), a family of peptides with antimicrobial and cytotoxic activity, in oral tumor tissue. Therefore, tissue sections were embedded in paraffin, and defensin was immunostained by the streptavidin-biotin coupled peroxidase method. Langerhans cells were confirmed by indirect immunostaining with anti-S-100 protein polyclonal antibody. Melanocytes were stained with Fontana-Masson's stain. Neurotrophins and intimal cells were stained by anti-defensin antibody. Langerhans cells in normal epithelium or dysplasic epithelium adjacent to squamous cell carcinoma and precancerous lesion were also stained. Defensins (HNP(s)) are nonspecific peptides that occur in neutrophils and protect against bacteria, fungi, and tumor cells. Since defensins are also found in epithelial Langerhans cells adjacent to tumor tissue, these peptides most likely have a role in tumor ummunity.

Original languageEnglish
Pages (from-to)2969-2971
Number of pages3
JournalAnticancer Research
Volume19
Issue number4 B
Publication statusPublished - 1999

Fingerprint

Defensins
Langerhans Cells
Epithelial Cells
Carcinoma
Peptides
Neoplasms
Epithelium
Tunica Intima
Streptavidin
S100 Proteins
Melanocytes
Nerve Growth Factors
Biotin
Paraffin
Peroxidase
Anti-Idiotypic Antibodies
Squamous Cell Carcinoma
Neutrophils
Fungi
Bacteria

Keywords

  • Defensin
  • HNP-1
  • Langerhans cells
  • Oral carcinoma precancerous lesion

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Presence of defensin in epithelial Langerhans cells adjacent to oral carcinomas and precancerous lesions. / Mizukawa, Nobuyoshi; Sugiyama, Katsumi; Yamachika, Eiki; Ueno, Takaaki; Mishima, Katsuaki; Takagi, Shin; Sugahara, Toshio.

In: Anticancer Research, Vol. 19, No. 4 B, 1999, p. 2969-2971.

Research output: Contribution to journalArticle

Mizukawa, N, Sugiyama, K, Yamachika, E, Ueno, T, Mishima, K, Takagi, S & Sugahara, T 1999, 'Presence of defensin in epithelial Langerhans cells adjacent to oral carcinomas and precancerous lesions', Anticancer Research, vol. 19, no. 4 B, pp. 2969-2971.
Mizukawa, Nobuyoshi ; Sugiyama, Katsumi ; Yamachika, Eiki ; Ueno, Takaaki ; Mishima, Katsuaki ; Takagi, Shin ; Sugahara, Toshio. / Presence of defensin in epithelial Langerhans cells adjacent to oral carcinomas and precancerous lesions. In: Anticancer Research. 1999 ; Vol. 19, No. 4 B. pp. 2969-2971.
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