Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis

Mitsuhiro Okano, Tazuko Fujiwara, Yuji Sugata, Daisuke Gotoh, Yoshihisa Masaoka, Masahiro Sogo, Wakana Tanimoto, Miki Yamamoto, Rie Matsumoto, Naomi Eguchi, Mamoru Kiniwa, Abdulcemal Umit Isik, Yoshihiro Urade, Kazunori Nishizaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Prostaglandin D2 (PGD2) is the major prostanoid produced in the acute phase of allergic reactions. However, its pathophysiological role in addition to the pathway of production in allergic rhinitis remains unclear. We sought to determine the expression of synthases and receptors for PGD2 in human nasal mucosa. These expressions were compared between allergic and nonallergic patients. Methods: The expres sion and localization of hematopoietic-type (h)-PGD2 synthase (PGDS) and lipocalin-type (l)-PGDS were detected by immunohistochemistry. The expression of D prostanoid (DP) receptor and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) was determined by quantitative real-time PCR. Results: The h-PG DS but not l-PGDS was clearly expressed in nasal mucosa. The expression of h-PGDS in allergic patients was significantly higher than in control patients without mucosal hypertrophy. A variety of infiltrating cells including mast cells, eosinophils, macrophages, and lymphocytes as well as constitutive cells such as epithelial cells and fibroblasts expressed h-PGDS. The expression of both DP and CRTH2 was confirmed also. Although either the amount of DP or the amount of CRTH2 was not correlated with serum levels of IgE, the amount of CRTH2 but not DP was highly and significantly correlated with the number of eosinophils infiltrating into nasal musosa. Conclusion: These results suggest that PGD2 is released via the action of h-PGDS from various cells, and the expression of h-PGDS may be associated with the hypertrophic inflammation in the nose. In addition, ligation of PGD2 to CRTH2 appears to be selectively involved in eosinophil recruitment into the nose regardless of atopic status.

Original languageEnglish
Pages (from-to)342-348
Number of pages7
JournalAmerican Journal of Rhinology
Volume20
Issue number3
DOIs
Publication statusPublished - May 2006

Fingerprint

prostaglandin R2 D-isomerase
Prostaglandin D2
Nasal Mucosa
Prostaglandins
Nose
Eosinophils
Formyl Peptide Receptor
Lipocalins
Th2 Cells
Acute-Phase Reaction
Allergic Rhinitis
Mast Cells
Immunoglobulin E
Hypertrophy
Ligation
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis. / Okano, Mitsuhiro; Fujiwara, Tazuko; Sugata, Yuji; Gotoh, Daisuke; Masaoka, Yoshihisa; Sogo, Masahiro; Tanimoto, Wakana; Yamamoto, Miki; Matsumoto, Rie; Eguchi, Naomi; Kiniwa, Mamoru; Isik, Abdulcemal Umit; Urade, Yoshihiro; Nishizaki, Kazunori.

In: American Journal of Rhinology, Vol. 20, No. 3, 05.2006, p. 342-348.

Research output: Contribution to journalArticle

Okano, M, Fujiwara, T, Sugata, Y, Gotoh, D, Masaoka, Y, Sogo, M, Tanimoto, W, Yamamoto, M, Matsumoto, R, Eguchi, N, Kiniwa, M, Isik, AU, Urade, Y & Nishizaki, K 2006, 'Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis', American Journal of Rhinology, vol. 20, no. 3, pp. 342-348. https://doi.org/10.2500/ajr.2006.20.2865
Okano, Mitsuhiro ; Fujiwara, Tazuko ; Sugata, Yuji ; Gotoh, Daisuke ; Masaoka, Yoshihisa ; Sogo, Masahiro ; Tanimoto, Wakana ; Yamamoto, Miki ; Matsumoto, Rie ; Eguchi, Naomi ; Kiniwa, Mamoru ; Isik, Abdulcemal Umit ; Urade, Yoshihiro ; Nishizaki, Kazunori. / Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis. In: American Journal of Rhinology. 2006 ; Vol. 20, No. 3. pp. 342-348.
@article{b20e1ff0537644659d728e6865f4d315,
title = "Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis",
abstract = "Background: Prostaglandin D2 (PGD2) is the major prostanoid produced in the acute phase of allergic reactions. However, its pathophysiological role in addition to the pathway of production in allergic rhinitis remains unclear. We sought to determine the expression of synthases and receptors for PGD2 in human nasal mucosa. These expressions were compared between allergic and nonallergic patients. Methods: The expres sion and localization of hematopoietic-type (h)-PGD2 synthase (PGDS) and lipocalin-type (l)-PGDS were detected by immunohistochemistry. The expression of D prostanoid (DP) receptor and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) was determined by quantitative real-time PCR. Results: The h-PG DS but not l-PGDS was clearly expressed in nasal mucosa. The expression of h-PGDS in allergic patients was significantly higher than in control patients without mucosal hypertrophy. A variety of infiltrating cells including mast cells, eosinophils, macrophages, and lymphocytes as well as constitutive cells such as epithelial cells and fibroblasts expressed h-PGDS. The expression of both DP and CRTH2 was confirmed also. Although either the amount of DP or the amount of CRTH2 was not correlated with serum levels of IgE, the amount of CRTH2 but not DP was highly and significantly correlated with the number of eosinophils infiltrating into nasal musosa. Conclusion: These results suggest that PGD2 is released via the action of h-PGDS from various cells, and the expression of h-PGDS may be associated with the hypertrophic inflammation in the nose. In addition, ligation of PGD2 to CRTH2 appears to be selectively involved in eosinophil recruitment into the nose regardless of atopic status.",
author = "Mitsuhiro Okano and Tazuko Fujiwara and Yuji Sugata and Daisuke Gotoh and Yoshihisa Masaoka and Masahiro Sogo and Wakana Tanimoto and Miki Yamamoto and Rie Matsumoto and Naomi Eguchi and Mamoru Kiniwa and Isik, {Abdulcemal Umit} and Yoshihiro Urade and Kazunori Nishizaki",
year = "2006",
month = "5",
doi = "10.2500/ajr.2006.20.2865",
language = "English",
volume = "20",
pages = "342--348",
journal = "American Journal of Rhinology and Allergy",
issn = "1945-8924",
publisher = "OceanSide Publications Inc.",
number = "3",

}

TY - JOUR

T1 - Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis

AU - Okano, Mitsuhiro

AU - Fujiwara, Tazuko

AU - Sugata, Yuji

AU - Gotoh, Daisuke

AU - Masaoka, Yoshihisa

AU - Sogo, Masahiro

AU - Tanimoto, Wakana

AU - Yamamoto, Miki

AU - Matsumoto, Rie

AU - Eguchi, Naomi

AU - Kiniwa, Mamoru

AU - Isik, Abdulcemal Umit

AU - Urade, Yoshihiro

AU - Nishizaki, Kazunori

PY - 2006/5

Y1 - 2006/5

N2 - Background: Prostaglandin D2 (PGD2) is the major prostanoid produced in the acute phase of allergic reactions. However, its pathophysiological role in addition to the pathway of production in allergic rhinitis remains unclear. We sought to determine the expression of synthases and receptors for PGD2 in human nasal mucosa. These expressions were compared between allergic and nonallergic patients. Methods: The expres sion and localization of hematopoietic-type (h)-PGD2 synthase (PGDS) and lipocalin-type (l)-PGDS were detected by immunohistochemistry. The expression of D prostanoid (DP) receptor and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) was determined by quantitative real-time PCR. Results: The h-PG DS but not l-PGDS was clearly expressed in nasal mucosa. The expression of h-PGDS in allergic patients was significantly higher than in control patients without mucosal hypertrophy. A variety of infiltrating cells including mast cells, eosinophils, macrophages, and lymphocytes as well as constitutive cells such as epithelial cells and fibroblasts expressed h-PGDS. The expression of both DP and CRTH2 was confirmed also. Although either the amount of DP or the amount of CRTH2 was not correlated with serum levels of IgE, the amount of CRTH2 but not DP was highly and significantly correlated with the number of eosinophils infiltrating into nasal musosa. Conclusion: These results suggest that PGD2 is released via the action of h-PGDS from various cells, and the expression of h-PGDS may be associated with the hypertrophic inflammation in the nose. In addition, ligation of PGD2 to CRTH2 appears to be selectively involved in eosinophil recruitment into the nose regardless of atopic status.

AB - Background: Prostaglandin D2 (PGD2) is the major prostanoid produced in the acute phase of allergic reactions. However, its pathophysiological role in addition to the pathway of production in allergic rhinitis remains unclear. We sought to determine the expression of synthases and receptors for PGD2 in human nasal mucosa. These expressions were compared between allergic and nonallergic patients. Methods: The expres sion and localization of hematopoietic-type (h)-PGD2 synthase (PGDS) and lipocalin-type (l)-PGDS were detected by immunohistochemistry. The expression of D prostanoid (DP) receptor and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) was determined by quantitative real-time PCR. Results: The h-PG DS but not l-PGDS was clearly expressed in nasal mucosa. The expression of h-PGDS in allergic patients was significantly higher than in control patients without mucosal hypertrophy. A variety of infiltrating cells including mast cells, eosinophils, macrophages, and lymphocytes as well as constitutive cells such as epithelial cells and fibroblasts expressed h-PGDS. The expression of both DP and CRTH2 was confirmed also. Although either the amount of DP or the amount of CRTH2 was not correlated with serum levels of IgE, the amount of CRTH2 but not DP was highly and significantly correlated with the number of eosinophils infiltrating into nasal musosa. Conclusion: These results suggest that PGD2 is released via the action of h-PGDS from various cells, and the expression of h-PGDS may be associated with the hypertrophic inflammation in the nose. In addition, ligation of PGD2 to CRTH2 appears to be selectively involved in eosinophil recruitment into the nose regardless of atopic status.

UR - http://www.scopus.com/inward/record.url?scp=33746114247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746114247&partnerID=8YFLogxK

U2 - 10.2500/ajr.2006.20.2865

DO - 10.2500/ajr.2006.20.2865

M3 - Article

C2 - 16871941

AN - SCOPUS:33746114247

VL - 20

SP - 342

EP - 348

JO - American Journal of Rhinology and Allergy

JF - American Journal of Rhinology and Allergy

SN - 1945-8924

IS - 3

ER -