Prepubertal Changes in Immunoreactive Inhibin Concentration in Blood Serum and Testicular Tissue in Holstein Bull Calves

Sigenori Matsuzaki, Yoshihisa Uenoyama, Kiyoshi Okuda, Gen Watanabe, Nobuo Kitamura, Kazuyoshi Taya, Maria Bella C. Cruzana, Junzo Yamada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Age-related changes in immunoreactive inhibin (ir-inhibin) levels and the relationship among ir-inhibin, gonadotropins and testosterone were examined in 53 Holstein bull calves from neonates to 8.6 months old. Serum levels of ir-inhibin, luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone, as well as ir-inhibin levels in testicular extracts, and testicular sizes were measured. All hormones were measured by specific radioimmunoassays. The concentrations of ir-inhibin in serum and testicular tissue were high in neonatal calves and tended to decrease with age. In contrast, serum concentrations of gonadotropins did not show any age-related changes within the experimental period. Serum testosterone levels and testicular sizes (length, width and weight) were positively correlated with age. Furthermore, a positive immunostaining to antiserum for the inhibin α-subunit was immunocytochemically observed only in Sertoli cells of the seminiferous tubules from neonates to calves less than 6 months old. These results indicate that the immature bovine testis produces and secretes high levels of ir-inhibin and that the Sertoli cells are a major source of ir-inhibin in prepubertal bull calves.

Original languageEnglish
Pages (from-to)1303-1307
Number of pages5
JournalJournal of Veterinary Medical Science
Volume63
Issue number12
DOIs
Publication statusPublished - Dec 1 2001

Keywords

  • Bull
  • Gonadotropin
  • Inhibin
  • Sertoli cell
  • Testosterone

ASJC Scopus subject areas

  • veterinary(all)

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    Matsuzaki, S., Uenoyama, Y., Okuda, K., Watanabe, G., Kitamura, N., Taya, K., Cruzana, M. B. C., & Yamada, J. (2001). Prepubertal Changes in Immunoreactive Inhibin Concentration in Blood Serum and Testicular Tissue in Holstein Bull Calves. Journal of Veterinary Medical Science, 63(12), 1303-1307. https://doi.org/10.1292/jvms.63.1303