Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera

Hideaki Takeuchi, Akikazu Yasuda, Yoshimi Yasuda-Kamatani, Miyuki Sawata, Yuko Matsuo, Azusa Kato, Atsumi Tsujimoto, Terumi Nakajima, Takeo Kubo

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Abstract

We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression in the honeybee brain by using in situ hybridization. The prepro-AmTRP gene is expressed predominantly in the MBs and in some neurons located in the optic and antennal lobes. cDNA microarray studies have revealed that AmTRP expression is enriched in the MBs compared with other brain regions. There is no difference in AmTRP-expressing cells among worker, queen, and drone brains, suggesting that the cell types that express the prepro-AmTRP gene do not change according to division of labor, sex, or caste. The unique expression pattern of the prepro-AmTRP gene suggests that AmTRPs function as neuromodulators in the MBs of the honeybee brain.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalCell and Tissue Research
Volume316
Issue number2
DOIs
Publication statusPublished - May 2004
Externally publishedYes

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Keywords

  • cDNA microarray
  • Honeybee, Apis mellifera (insecta)
  • Mushroom body
  • Social behavior
  • Tackykinin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Sawata, M., Matsuo, Y., Kato, A., Tsujimoto, A., Nakajima, T., & Kubo, T. (2004). Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera. Cell and Tissue Research, 316(2), 281-293. https://doi.org/10.1007/s00441-004-0865-y