Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery

Tsuyoshi Kimura, Sayaka Iwai, Toshiyuki Moritan, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Tosihya Fujisato, Akio Kishida

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Poly(vinyl alcohol) (PVA) hydrogels interacting with DNA mediated by hydrogen bonds (PVA/DNA hydrogel) were developed using ultra-high pressure (UHP) technology. The goal was to create a new method of gene delivery by controlled release of DNA. Mixed solutions of DNA and PVA at various concentrations were pressurized at 10 000 atmospheres at 37°C for 10 min. PVA/DNA hydrogels with good formability were produced at PVA concentrations of more than 5% w/v. The presence of DNA in the obtained hydrogels was confirmed by spectroscopic analysis and nucleic acid dye staining. DNA release from the hydrogels was investigated using PVA/DNA hydrogel samples of 5% and 10% w/v formed by UHP treatment or by conventional freeze-thaw methods. The DNA release curves from both types of samples showed a rapid phase in the initial 15 h followed by a sustained release phase. However, there was a difference in the amount of DNA released. Less DNA was released by the pressurized hydrogels than by the freeze-thaw hydrogels. Also, the cumulative amount of DNA released decreased as the PVA content in the hydrogels increased. These results indicate that DNA release from the hydrogels can be modulated by changing the preparation method and the PVA content. Furthermore, it was demonstrated that DNA release could be controlled by varying the amount and duration of pressurizing used to form the hydrogels. Intact fractions of plasmid DNA released from the hydrogels were separated by agarose gel electrophoretic analysis. These results suggest that, using controlled release, DNA from PVA/DNA hydrogels formed by UHP treatment can be transfected into cells.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalJournal of Artificial Organs
Volume10
Issue number2
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Hydrogels
Pressurization
Hydrogen
Hydrogen bonds
Alcohols
DNA
Genes
Hydrogel
Pressure
Spectroscopic analysis
Nucleic acids
Formability

Keywords

  • Controlled release
  • DNA
  • Hydrogel
  • Poly(vinyl alcohol)
  • Ultra-high pressure

ASJC Scopus subject areas

  • Biophysics

Cite this

Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery. / Kimura, Tsuyoshi; Iwai, Sayaka; Moritan, Toshiyuki; Nam, Kwangwoo; Mutsuo, Shingo; Yoshizawa, Hidekazu; Okada, Masahiro; Furuzono, Tsutomu; Fujisato, Tosihya; Kishida, Akio.

In: Journal of Artificial Organs, Vol. 10, No. 2, 06.2007, p. 104-108.

Research output: Contribution to journalArticle

Kimura, Tsuyoshi ; Iwai, Sayaka ; Moritan, Toshiyuki ; Nam, Kwangwoo ; Mutsuo, Shingo ; Yoshizawa, Hidekazu ; Okada, Masahiro ; Furuzono, Tsutomu ; Fujisato, Tosihya ; Kishida, Akio. / Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery. In: Journal of Artificial Organs. 2007 ; Vol. 10, No. 2. pp. 104-108.
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AU - Iwai, Sayaka

AU - Moritan, Toshiyuki

AU - Nam, Kwangwoo

AU - Mutsuo, Shingo

AU - Yoshizawa, Hidekazu

AU - Okada, Masahiro

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AB - Poly(vinyl alcohol) (PVA) hydrogels interacting with DNA mediated by hydrogen bonds (PVA/DNA hydrogel) were developed using ultra-high pressure (UHP) technology. The goal was to create a new method of gene delivery by controlled release of DNA. Mixed solutions of DNA and PVA at various concentrations were pressurized at 10 000 atmospheres at 37°C for 10 min. PVA/DNA hydrogels with good formability were produced at PVA concentrations of more than 5% w/v. The presence of DNA in the obtained hydrogels was confirmed by spectroscopic analysis and nucleic acid dye staining. DNA release from the hydrogels was investigated using PVA/DNA hydrogel samples of 5% and 10% w/v formed by UHP treatment or by conventional freeze-thaw methods. The DNA release curves from both types of samples showed a rapid phase in the initial 15 h followed by a sustained release phase. However, there was a difference in the amount of DNA released. Less DNA was released by the pressurized hydrogels than by the freeze-thaw hydrogels. Also, the cumulative amount of DNA released decreased as the PVA content in the hydrogels increased. These results indicate that DNA release from the hydrogels can be modulated by changing the preparation method and the PVA content. Furthermore, it was demonstrated that DNA release could be controlled by varying the amount and duration of pressurizing used to form the hydrogels. Intact fractions of plasmid DNA released from the hydrogels were separated by agarose gel electrophoretic analysis. These results suggest that, using controlled release, DNA from PVA/DNA hydrogels formed by UHP treatment can be transfected into cells.

KW - Controlled release

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KW - Ultra-high pressure

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