Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection

Tsuyoshi Kimura, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Toshiya Fujisao, Akio Kishida

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have performed the development of nano-inorgenic particle/polymer/DNA composites by ultra high pressure technology for gene transfection. Nano scaled hydroxy apatite (HAp) was used as nano-inorgenic particle. Polyvinyl alcohol (PVA, 5, 7.5, 10w/v%) aqueous solution mixed with HAp(0.01-10mg/ml) and DNA, then treated by ultra high pressure at 10,000 atm for 10min. HAp/PVA/DNA hydrogel was obtained. By SEM observation, HAp particles on the surface of the hydrogel were observed. By DNA staining, the including of DNA in the hydrogel was confirmed. In order to investigate the cell affinity of the hydrogel, various cell lines were added on the hydrogels. In the case of PVA/DNA hydrogel, almost all of cells were not adhered. On the other hand, the effective cell adhesion was observed on HAp/PVA/DNA hydrogel.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2055
Number of pages1
Volume55
Edition1
Publication statusPublished - 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

Inorganic polymers
Hydrogels
DNA
Genes
Hydroxyapatite
Polyvinyl alcohols
Cell adhesion
Particles (particulate matter)
Cells
Scanning electron microscopy
Composite materials
Polymers

Keywords

  • Hydrogel
  • Hydroxy apatite
  • Transfection
  • Ultra high pressure treatment

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kimura, T., Nam, K., Mutsuo, S., Yoshizawa, H., Okada, M., Furuzono, T., ... Kishida, A. (2006). Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. In Polymer Preprints, Japan (1 ed., Vol. 55, pp. 2055)

Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. / Kimura, Tsuyoshi; Nam, Kwangwoo; Mutsuo, Shingo; Yoshizawa, Hidekazu; Okada, Masahiro; Furuzono, Tsutomu; Fujisao, Toshiya; Kishida, Akio.

Polymer Preprints, Japan. Vol. 55 1. ed. 2006. p. 2055.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kimura, T, Nam, K, Mutsuo, S, Yoshizawa, H, Okada, M, Furuzono, T, Fujisao, T & Kishida, A 2006, Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. in Polymer Preprints, Japan. 1 edn, vol. 55, pp. 2055, 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06.
Kimura T, Nam K, Mutsuo S, Yoshizawa H, Okada M, Furuzono T et al. Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. In Polymer Preprints, Japan. 1 ed. Vol. 55. 2006. p. 2055
Kimura, Tsuyoshi ; Nam, Kwangwoo ; Mutsuo, Shingo ; Yoshizawa, Hidekazu ; Okada, Masahiro ; Furuzono, Tsutomu ; Fujisao, Toshiya ; Kishida, Akio. / Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. Polymer Preprints, Japan. Vol. 55 1. ed. 2006. pp. 2055
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