Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection

Tsuyoshi Kimura, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Toshiya Fujisao, Akio Kishida

Research output: Contribution to conferencePaper

Abstract

We have performed the development of nano-inorgenic particle/polymer/DNA composites by ultra high pressure technology for gene transfection. Nano scaled hydroxy apatite (HAp) was used as nano-inorgenic particle. Polyvinyl alcohol (PVA, 5, 7.5, 10w/v%) aqueous solution mixed with HAp(0.01-10mg/ml) and DNA, then treated by ultra high pressure at 10,000 atm for 10min. HAp/PVA/DNA hydrogel was obtained. By SEM observation, HAp particles on the surface of the hydrogel were observed. By DNA staining, the including of DNA in the hydrogel was confirmed. In order to investigate the cell affinity of the hydrogel, various cell lines were added on the hydrogels. In the case of PVA/DNA hydrogel, almost all of cells were not adhered. On the other hand, the effective cell adhesion was observed on HAp/PVA/DNA hydrogel.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Keywords

  • Hydrogel
  • Hydroxy apatite
  • Transfection
  • Ultra high pressure treatment

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Kimura, T., Nam, K., Mutsuo, S., Yoshizawa, H., Okada, M., Furuzono, T., Fujisao, T., & Kishida, A. (2006). Preparation of nano-inorganic particel/polymer/DNA compsites by ultra high pressure technology for gene transfection. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.