Preparation of Mammalian Cell-Enclosing Subsieve-Sized Capsules (< 100 μm) in a Coflowing Stream

Shinji Sakai, Kenji Kawabata, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The droplet breakup technique with an immiscible liquid coflowing stream was investigated for the preparation of mammalian cell-enclosing subsieve-sized capsules of less than 100 μm in diameter. The major parts of the droplet generation device were a needle of several hundred micrometers in diameter for extruding the cell-suspending sodium alginate aqueous solution and a tubule of 2.5 mm in diameter through which the extruded alginate solution flowed into ambient immiscible liquid paraffin. The needle was positioned upstream in the vicinity of the coaxial tubule. The droplet diameter of the viscous sodium alginate aqueous solution could be controlled from several dozen to several hundred micrometers by changing the velocities of the inner and ambient fluids and the diameter of the needle. By utilizing a 300-μm diameter needle, CHO-K1 cell-enclosing droplets of 48 ± 8 μm in diameter were obtained by extruding a cell-suspending sodium alginate solution at a velocity of 1.2 cm/sec into the ambient liquid paraffin flowing at a velocity of 23.5 cm/sec. The breakup process did not influence the viability of the enclosed cells, since more than 95% of the CHO-K1 cells remained alive after the enclosing process.

Original languageEnglish
Pages (from-to)168-173
Number of pages6
JournalBiotechnology and Bioengineering
Volume86
Issue number2
DOIs
Publication statusPublished - Apr 20 2004
Externally publishedYes

Keywords

  • Alginate
  • Cell therapy
  • Coflowing stream
  • Laminar flow
  • Microcapsule
  • Subsieve-sized capsule

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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