Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction

Yuki Shirosaki, Satoshi Hayakawa, Akiyoshi Osaka, Maria A. Lopes, José D. Santos, Ana C. Maurîcio, Stefano Geuna

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The treatment of peripheral nerve injuries is still one of the most challenging tasks in neurosurgery, as functional recovery is rarely satisfactory in these patients. The concept behind the use of biodegradable nerve guides is that no foreign material should be left in place after the device has fulfilled its task, so as to spare a second surgical intervention. In a previous study, flexible and biodegradable chitosan-3-glycidoxypropyltrimethoxysilane (GPTMS) hybrid membranes exhibited better cytocompatibility in terms of osteoblastic cells than chitosan membrane. Porous chitosan hybrid membranes, derived by freeze-drying the hybrid gels, showed that the cells were attached and proliferated both on the surface and into pores. In this study, the porous chitosan-silicate hybrid membranes with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord were used to reconstruct the crushed peripheral nerve. Axonotmesis lesion of 3 mm was enwrapped with the hybrid membranes with or without a monolayer of non-differentiated human mesenchymal stem cells. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks. The combination of hybrid membranes and human MSCs infiltration showed a slightly better recovery than the untreated control (the just crushed nerve). On the other hand, the hybrid membrane alone promoted the crushed peripheral nerve reconstruction. It is expected that the porous chitosan hybrid membranes is candidate for the clinical tool in peripheral nerve reconstructive surgery.

Original languageEnglish
Title of host publication9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013
PublisherIMAPS-International Microelectronics and Packaging Society
Pages173-176
Number of pages4
ISBN (Print)9781629937182
Publication statusPublished - 2013
Event9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013 - Orlando, FL, United States
Duration: Apr 23 2013Apr 25 2013

Other

Other9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013
CountryUnited States
CityOrlando, FL
Period4/23/134/25/13

Fingerprint

Membranes
Chitosan
Stem cells
Recovery
Neurosurgery
Infiltration
Surgery
Silicates
Monolayers
Drying
Gels

Keywords

  • Chitosan
  • Membrane
  • Nerve regeneration
  • Organic-inorganic hybrid

ASJC Scopus subject areas

  • Hardware and Architecture
  • Control and Systems Engineering

Cite this

Shirosaki, Y., Hayakawa, S., Osaka, A., Lopes, M. A., Santos, J. D., Maurîcio, A. C., & Geuna, S. (2013). Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction. In 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013 (pp. 173-176). IMAPS-International Microelectronics and Packaging Society.

Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction. / Shirosaki, Yuki; Hayakawa, Satoshi; Osaka, Akiyoshi; Lopes, Maria A.; Santos, José D.; Maurîcio, Ana C.; Geuna, Stefano.

9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013. IMAPS-International Microelectronics and Packaging Society, 2013. p. 173-176.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirosaki, Y, Hayakawa, S, Osaka, A, Lopes, MA, Santos, JD, Maurîcio, AC & Geuna, S 2013, Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction. in 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013. IMAPS-International Microelectronics and Packaging Society, pp. 173-176, 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013, Orlando, FL, United States, 4/23/13.
Shirosaki Y, Hayakawa S, Osaka A, Lopes MA, Santos JD, Maurîcio AC et al. Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction. In 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013. IMAPS-International Microelectronics and Packaging Society. 2013. p. 173-176
Shirosaki, Yuki ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Lopes, Maria A. ; Santos, José D. ; Maurîcio, Ana C. ; Geuna, Stefano. / Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction. 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013. IMAPS-International Microelectronics and Packaging Society, 2013. pp. 173-176
@inproceedings{0ace1573f61247b798a76d39230b5867,
title = "Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction",
abstract = "The treatment of peripheral nerve injuries is still one of the most challenging tasks in neurosurgery, as functional recovery is rarely satisfactory in these patients. The concept behind the use of biodegradable nerve guides is that no foreign material should be left in place after the device has fulfilled its task, so as to spare a second surgical intervention. In a previous study, flexible and biodegradable chitosan-3-glycidoxypropyltrimethoxysilane (GPTMS) hybrid membranes exhibited better cytocompatibility in terms of osteoblastic cells than chitosan membrane. Porous chitosan hybrid membranes, derived by freeze-drying the hybrid gels, showed that the cells were attached and proliferated both on the surface and into pores. In this study, the porous chitosan-silicate hybrid membranes with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord were used to reconstruct the crushed peripheral nerve. Axonotmesis lesion of 3 mm was enwrapped with the hybrid membranes with or without a monolayer of non-differentiated human mesenchymal stem cells. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks. The combination of hybrid membranes and human MSCs infiltration showed a slightly better recovery than the untreated control (the just crushed nerve). On the other hand, the hybrid membrane alone promoted the crushed peripheral nerve reconstruction. It is expected that the porous chitosan hybrid membranes is candidate for the clinical tool in peripheral nerve reconstructive surgery.",
keywords = "Chitosan, Membrane, Nerve regeneration, Organic-inorganic hybrid",
author = "Yuki Shirosaki and Satoshi Hayakawa and Akiyoshi Osaka and Lopes, {Maria A.} and Santos, {Jos{\'e} D.} and Maur{\^i}cio, {Ana C.} and Stefano Geuna",
year = "2013",
language = "English",
isbn = "9781629937182",
pages = "173--176",
booktitle = "9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013",
publisher = "IMAPS-International Microelectronics and Packaging Society",

}

TY - GEN

T1 - Preparation of inorganic-organic hybrid membrane for peripheral nerve reconstruction

AU - Shirosaki, Yuki

AU - Hayakawa, Satoshi

AU - Osaka, Akiyoshi

AU - Lopes, Maria A.

AU - Santos, José D.

AU - Maurîcio, Ana C.

AU - Geuna, Stefano

PY - 2013

Y1 - 2013

N2 - The treatment of peripheral nerve injuries is still one of the most challenging tasks in neurosurgery, as functional recovery is rarely satisfactory in these patients. The concept behind the use of biodegradable nerve guides is that no foreign material should be left in place after the device has fulfilled its task, so as to spare a second surgical intervention. In a previous study, flexible and biodegradable chitosan-3-glycidoxypropyltrimethoxysilane (GPTMS) hybrid membranes exhibited better cytocompatibility in terms of osteoblastic cells than chitosan membrane. Porous chitosan hybrid membranes, derived by freeze-drying the hybrid gels, showed that the cells were attached and proliferated both on the surface and into pores. In this study, the porous chitosan-silicate hybrid membranes with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord were used to reconstruct the crushed peripheral nerve. Axonotmesis lesion of 3 mm was enwrapped with the hybrid membranes with or without a monolayer of non-differentiated human mesenchymal stem cells. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks. The combination of hybrid membranes and human MSCs infiltration showed a slightly better recovery than the untreated control (the just crushed nerve). On the other hand, the hybrid membrane alone promoted the crushed peripheral nerve reconstruction. It is expected that the porous chitosan hybrid membranes is candidate for the clinical tool in peripheral nerve reconstructive surgery.

AB - The treatment of peripheral nerve injuries is still one of the most challenging tasks in neurosurgery, as functional recovery is rarely satisfactory in these patients. The concept behind the use of biodegradable nerve guides is that no foreign material should be left in place after the device has fulfilled its task, so as to spare a second surgical intervention. In a previous study, flexible and biodegradable chitosan-3-glycidoxypropyltrimethoxysilane (GPTMS) hybrid membranes exhibited better cytocompatibility in terms of osteoblastic cells than chitosan membrane. Porous chitosan hybrid membranes, derived by freeze-drying the hybrid gels, showed that the cells were attached and proliferated both on the surface and into pores. In this study, the porous chitosan-silicate hybrid membranes with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord were used to reconstruct the crushed peripheral nerve. Axonotmesis lesion of 3 mm was enwrapped with the hybrid membranes with or without a monolayer of non-differentiated human mesenchymal stem cells. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks. The combination of hybrid membranes and human MSCs infiltration showed a slightly better recovery than the untreated control (the just crushed nerve). On the other hand, the hybrid membrane alone promoted the crushed peripheral nerve reconstruction. It is expected that the porous chitosan hybrid membranes is candidate for the clinical tool in peripheral nerve reconstructive surgery.

KW - Chitosan

KW - Membrane

KW - Nerve regeneration

KW - Organic-inorganic hybrid

UR - http://www.scopus.com/inward/record.url?scp=84901953815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901953815&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84901953815

SN - 9781629937182

SP - 173

EP - 176

BT - 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013

PB - IMAPS-International Microelectronics and Packaging Society

ER -