Preparation of hydroxyapatite-nanocrystal-coated stainless steel, and its cell interaction

Masahiro Okada, Miwa Masuda, Ryoichi Tanaka, Kunio Miyatake, Daisuke Kuroda, Tsutomu Furuzono

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Calcined nanocrystals of hydroxyapatite (HAp) having spherical or rod-shaped morphologies were coated through covalent linkage on a type 316L stainless steel substrate, which was chemically modified by the graft polymerization of γ-methacryloxypropyl triethoxysilane (MPTS) at 70-110°C. The grafting of poly(MPTS) on the substrate was confirmed by X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR). In order to coat the substrate with the HAp crystals through covalent linkage, the reaction between the alkoxysilyl groups in the poly(MPTS) grafted on the substrate and the OH groups on the HAp crystals was conducted at 80°C. The poly(MPTS)-grafted substrate was strongly coated with the HAp nanocrystals, although the HAp crystals adsorbed physically on the original substrate without poly(MPTS) grafting were removed by ultrasonic treatment. Human umbilical vein endothelial cells (HUVEC) adhered in larger numbers on the HAp-coated stainless steel substrate as compared with the original substrate after 24 h of initial incubation. The number of HUVEC adhered on the rod-shaped HAp-coated substrate was not significantly different from that on the spherical HAp-coated substrate under the present conditions.

Original languageEnglish
Pages (from-to)589-596
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume86
Issue number3
DOIs
Publication statusPublished - Sep 1 2008
Externally publishedYes

Keywords

  • Cell adhesion
  • Composite
  • Covalent linkage
  • Hydroxyapatite
  • Nano-sized crystal

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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