TY - JOUR
T1 - Preparation of fine W/O/W emulsion through membrane filtration of coarse W/O/W emulsion and disappearance of the inclusion of outer phase solution
AU - Shima, Motohiro
AU - Kobayashi, Yohei
AU - Fujii, Takao
AU - Tanaka, Miyono
AU - Kimura, Yukitaka
AU - Adachi, Shuji
AU - Matsuno, Ryuichi
N1 - Funding Information:
This work was supported by a grant-in-aid for scientific research from the Japan Society of the Promotion of Science and from the Program for the Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN), Japan. We thank Prof. Suzuki for his instruction on the membrane-filtration method. The surfactants, SY-GLYSTER ® ML-750 and Sunsoft ® 818SX, were gifts from Sakamoto Yakuhin Kogyo (Osaka, Japan) and Taiyo Kagaku (Yokkaichi, Japan), respectively.
PY - 2004/1
Y1 - 2004/1
N2 - A water-in-oil-in-water (W/O/W) emulsion was prepared as a carrier system for the daily uptake of a bioactive compound using decaglycerol monolaurate and hexaglyceryl condensed ricinolate as hydrophilic and lipophilic surfactants, respectively. 1,3,6,8-Pyrenetetrasulfonic acid tetrasodium salt (PTSA) was used as a hydrophilic model compound of a bioactive substance. Membrane filtration of a coarse W/O/W emulsion prepared with a rotor/stator homogenizer produced a fine emulsion which had a mean oil-droplet diameter of < 1 μm. The emulsions were stable for 1 week at 4 °C. The encapsulation efficiency of PTSA was >90%. Microscopic observations revealed that the fluorescent material in the outer-phase solution was included into the oil phase during rotor/stator homogenization. It was suggested that because the rotor/stator homogenizer was used to produce both O/W and W/O/W emulsions, some part of the outer phase solution would be included in the oil phase during the second homogenization. The fluorescence shown in the oil droplets of the coarse W/O/W emulsion was diminished after membrane emulsification. This phenomenon suggested that the included outer-phase solution was released to the outer-phase solution from the interior of the W/O/W oil droplets during the membrane emulsification.
AB - A water-in-oil-in-water (W/O/W) emulsion was prepared as a carrier system for the daily uptake of a bioactive compound using decaglycerol monolaurate and hexaglyceryl condensed ricinolate as hydrophilic and lipophilic surfactants, respectively. 1,3,6,8-Pyrenetetrasulfonic acid tetrasodium salt (PTSA) was used as a hydrophilic model compound of a bioactive substance. Membrane filtration of a coarse W/O/W emulsion prepared with a rotor/stator homogenizer produced a fine emulsion which had a mean oil-droplet diameter of < 1 μm. The emulsions were stable for 1 week at 4 °C. The encapsulation efficiency of PTSA was >90%. Microscopic observations revealed that the fluorescent material in the outer-phase solution was included into the oil phase during rotor/stator homogenization. It was suggested that because the rotor/stator homogenizer was used to produce both O/W and W/O/W emulsions, some part of the outer phase solution would be included in the oil phase during the second homogenization. The fluorescence shown in the oil droplets of the coarse W/O/W emulsion was diminished after membrane emulsification. This phenomenon suggested that the included outer-phase solution was released to the outer-phase solution from the interior of the W/O/W oil droplets during the membrane emulsification.
KW - Bioactive compound
KW - Fine water-in-oil-in-water emulsion
KW - Functional food
KW - Membrane emulsification
KW - Rotor/stator homogenizer
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U2 - 10.1016/S0268-005X(03)00042-0
DO - 10.1016/S0268-005X(03)00042-0
M3 - Article
AN - SCOPUS:0344013445
SN - 0268-005X
VL - 18
SP - 61
EP - 70
JO - Food Hydrocolloids
JF - Food Hydrocolloids
IS - 1
ER -