Preparation of alginic acid layers on stainless-steel substrates for biomedical applications

Tomohiko Yoshioka, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

This study is concerned with the blood compatibility of alginic acid layers immobilized on γ-aminopropyltriethoxysilane (γ-APS)-grafted stainless-steel (SUS316L). The surfaces were characterized with contact angle measurement and X-ray photoelectron spectroscopy (XPS). The blood compatibility was evaluated in terms of platelet adhesion and blood clotting time. An in vitro platelet adhesion assay indicated that only a small number of platelets adhered to substrate surfaces modified with γ-APS and subsequently with alginic acid. Moreover, alginic-acid-immobilized SUS316L substrates had little effect on the blood clotting time. This indicated that alginic-acid-immobilized SUS316L substrates do not adsorb some blood-clotting proteins or factors, or stimulate them.

Original languageEnglish
Pages (from-to)2889-2894
Number of pages6
JournalBiomaterials
Volume24
Issue number17
DOIs
Publication statusPublished - Aug 2003

Fingerprint

Alginate
Stainless Steel
Blood Coagulation
Blood
Stainless steel
Platelets
Substrates
Blood Platelets
Photoelectron Spectroscopy
Adhesion
Platelet Count
Blood Proteins
Angle measurement
Contact angle
Assays
X ray photoelectron spectroscopy
alginic acid
Proteins
SUS 316L

Keywords

  • γ-Aminopropyltriethoxysilane
  • Alginic acid
  • Blood compatibility
  • Blood-clotting time
  • Platelet adhesion
  • Stainless steel

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Preparation of alginic acid layers on stainless-steel substrates for biomedical applications. / Yoshioka, Tomohiko; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi.

In: Biomaterials, Vol. 24, No. 17, 08.2003, p. 2889-2894.

Research output: Contribution to journalArticle

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