Preparation and protein adsorption of silica-based composite particles for blood purification therapy

Jie Li, Yuki Shirosaki, Satoshi Hayakawa, Akiyoshi Osaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Silica gel macrospheres of a few millimeters in diameter were fabricated via the sol-gel route using water glass, calcium chloride, and sodium alginate as the precursor components. TiO2 coating on the silica gel macrosphere surface was conducted by soaking them in titania sol derived from hydrolysis of tetraethylorthotitanate (TEOT) under varied pH (HNO3), and temperature (50-80°C). X-ray diffraction analysis indicated deposition of anatases, and particular deposition (<1μm) on the surface was confirmed by scanning electron microscopy. Protein adsorption behavior was examined as a function of pH of the hydrolysis of TEOT, using bovine serum albumin (BSA) and lysozyme (LSZ) as the model proteins. BSA adsorption was little affected by the pH, while some effects were found for LSZ adsorption. BSA adsorption saturated at 20-25 min contact, while LSZ adsorption took longer time for the saturation. Moreover, BSA was adsorbed almost twice as much as LSZ, when saturation adsorption was compared. An effect of residual calcium ions with better affinity to BSA was proposed for partial interpretation since surface charge could not account for those adsorption behaviors.

Original languageEnglish
Title of host publicationAdvances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11
Pages13-18
Number of pages6
Edition6
Publication statusPublished - Dec 2 2011
EventAdvances in Bioceramics and Porous Ceramics IV - 35th International Conference on Advanced Ceramics and Composites, ICACC'11 - Daytona Beach, FL, United States
Duration: Jan 23 2011Jan 28 2011

Publication series

NameCeramic Engineering and Science Proceedings
Number6
Volume32
ISSN (Print)0196-6219

Other

OtherAdvances in Bioceramics and Porous Ceramics IV - 35th International Conference on Advanced Ceramics and Composites, ICACC'11
CountryUnited States
CityDaytona Beach, FL
Period1/23/111/28/11

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

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  • Cite this

    Li, J., Shirosaki, Y., Hayakawa, S., & Osaka, A. (2011). Preparation and protein adsorption of silica-based composite particles for blood purification therapy. In Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11 (6 ed., pp. 13-18). (Ceramic Engineering and Science Proceedings; Vol. 32, No. 6).