Preparation and in vitro/in vivo evaluations of dimpled poly(L-lactic acid) fibers mixed/coated with hydroxyapatite nanocrystals

Hiroshi Yanagida, Masahiro Okada, Miwa Masuda, Isao Narama, Shigeyuki Nakano, Satoshi Kitao, Kazuo Takakuda, Tsutomu Furuzono

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A novel hydroxyapatite (HAp)/poly(l-lactic acid) (PLLA) nanocomposite nonwoven fabric, which was coated and mixed with calcined HAp nanocrystals, and has submicron-sized dimples on its surface, was fabricated. First, HAp-mixed PLLA fabric was prepared by electrospinning a HAp nanocrystal dispersion in dichloromethane (DCM)-dissolved PLLA. It was found that most of the HAp nanocrystals were not exposed on the HAp-mixed PLLA fiber surface but covered with the PLLA matrix. A HAp-nanocrystal coating was applied onto the surface of the HAp-mixed PLLA fabric after corona discharge treatment followed by ethanol washing. The submicron-sized dimples were enlarged after the ethanol washing. After the HAp-nanocrystal coating, the HAp-mixed PLLA fabric surface was uniformly coated with the HAp nanocrystals. In vitro cell spread tests showed that the rat osteoblasts spread more on HAp-nanocrystal-coated fabrics than on non-HAp-coated fabrics. Upon covering calvarial defects, the in vivo hard tissue responses suggested earlier restoration of the defects with HAp-nanocrystal-coated fabrics than those with non-HAp-coated fabrics.

Original languageEnglish
Pages (from-to)331-341
Number of pages11
JournalJournal of Artificial Organs
Volume14
Issue number4
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Fingerprint

Durapatite
Lactic acid
Hydroxyapatite
Nanoparticles
Nanocrystals
Fibers
Lactic Acid
Washing
poly(lactic acid)
In Vitro Techniques
Ethanol
Nanocomposites
Coatings
Defects
Nonwoven fabrics
Methylene Chloride
Osteoblasts
Dichloromethane
Electrospinning
Restoration

Keywords

  • Bone defect
  • Composite
  • Hydroxyapatite
  • Osteoblast
  • Poly(l-lactic acid)

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine
  • Medicine (miscellaneous)

Cite this

Preparation and in vitro/in vivo evaluations of dimpled poly(L-lactic acid) fibers mixed/coated with hydroxyapatite nanocrystals. / Yanagida, Hiroshi; Okada, Masahiro; Masuda, Miwa; Narama, Isao; Nakano, Shigeyuki; Kitao, Satoshi; Takakuda, Kazuo; Furuzono, Tsutomu.

In: Journal of Artificial Organs, Vol. 14, No. 4, 12.2011, p. 331-341.

Research output: Contribution to journalArticle

Yanagida, Hiroshi ; Okada, Masahiro ; Masuda, Miwa ; Narama, Isao ; Nakano, Shigeyuki ; Kitao, Satoshi ; Takakuda, Kazuo ; Furuzono, Tsutomu. / Preparation and in vitro/in vivo evaluations of dimpled poly(L-lactic acid) fibers mixed/coated with hydroxyapatite nanocrystals. In: Journal of Artificial Organs. 2011 ; Vol. 14, No. 4. pp. 331-341.
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