TY - JOUR
T1 - Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.
AU - Masaoka, A.
AU - Terato, H.
AU - Honsho, A.
AU - Ohyama, Y.
AU - Suzuki, T.
AU - Yamada, M.
AU - Makino, K.
AU - Ide, H.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2000
Y1 - 2000
N2 - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
AB - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
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U2 - 10.1093/nass/44.1.87
DO - 10.1093/nass/44.1.87
M3 - Article
C2 - 12903281
AN - SCOPUS:0141670285
SP - 87
EP - 88
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 44
ER -