Abstract
Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
| Original language | English |
|---|---|
| Pages (from-to) | 87-88 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series |
| Issue number | 44 |
| Publication status | Published - Jan 1 2000 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
Cite this
Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides. / Masaoka, A.; Terato, Hiroaki; Honsho, A.; Ohyama, Y.; Suzuki, T.; Yamada, M.; Makino, K.; Ide, H.
In: Nucleic acids symposium series, No. 44, 01.01.2000, p. 87-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.
AU - Masaoka, A.
AU - Terato, Hiroaki
AU - Honsho, A.
AU - Ohyama, Y.
AU - Suzuki, T.
AU - Yamada, M.
AU - Makino, K.
AU - Ide, H.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
AB - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
UR - http://www.scopus.com/inward/record.url?scp=0141670285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141670285&partnerID=8YFLogxK
M3 - Article
SP - 87
EP - 88
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 44
ER -