Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

A. Masaoka, H. Terato, A. Honsho, Y. Ohyama, T. Suzuki, M. Yamada, K. Makino, H. Ide

Research output: Contribution to journalArticlepeer-review

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Original languageEnglish
Pages (from-to)87-88
Number of pages2
JournalNucleic acids symposium series
Issue number44
DOIs
Publication statusPublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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