Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

A. Masaoka, Hiroaki Terato, A. Honsho, Y. Ohyama, T. Suzuki, M. Yamada, K. Makino, H. Ide

Research output: Contribution to journalArticle

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Original languageEnglish
Pages (from-to)87-88
Number of pages2
JournalNucleic acids symposium series
Issue number44
Publication statusPublished - Jan 1 2000
Externally publishedYes

Fingerprint

Nitrogen Oxides
Xanthine
Guanine
DNA Glycosylases
Deoxyribonucleosides
Deamination
DNA-Directed DNA Polymerase
Nucleotides
oxanine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Masaoka, A., Terato, H., Honsho, A., Ohyama, Y., Suzuki, T., Yamada, M., ... Ide, H. (2000). Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides. Nucleic acids symposium series, (44), 87-88.

Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides. / Masaoka, A.; Terato, Hiroaki; Honsho, A.; Ohyama, Y.; Suzuki, T.; Yamada, M.; Makino, K.; Ide, H.

In: Nucleic acids symposium series, No. 44, 01.01.2000, p. 87-88.

Research output: Contribution to journalArticle

Masaoka, A, Terato, H, Honsho, A, Ohyama, Y, Suzuki, T, Yamada, M, Makino, K & Ide, H 2000, 'Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.', Nucleic acids symposium series, no. 44, pp. 87-88.
Masaoka, A. ; Terato, Hiroaki ; Honsho, A. ; Ohyama, Y. ; Suzuki, T. ; Yamada, M. ; Makino, K. ; Ide, H. / Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides. In: Nucleic acids symposium series. 2000 ; No. 44. pp. 87-88.
@article{fc8154f98c2745afab270a6b2695d9c9,
title = "Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.",
abstract = "Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.",
author = "A. Masaoka and Hiroaki Terato and A. Honsho and Y. Ohyama and T. Suzuki and M. Yamada and K. Makino and H. Ide",
year = "2000",
month = "1",
day = "1",
language = "English",
pages = "87--88",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "44",

}

TY - JOUR

T1 - Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

AU - Masaoka, A.

AU - Terato, Hiroaki

AU - Honsho, A.

AU - Ohyama, Y.

AU - Suzuki, T.

AU - Yamada, M.

AU - Makino, K.

AU - Ide, H.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

AB - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

UR - http://www.scopus.com/inward/record.url?scp=0141670285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141670285&partnerID=8YFLogxK

M3 - Article

SP - 87

EP - 88

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 44

ER -