Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

A. Masaoka; H. Terato; A. Honsho; Y. Ohyama; T. Suzuki; M. Yamada; K. Makino; H. Ide

doi:10.1093/nass/44.1.87

Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

A. Masaoka, H. Terato, A. Honsho, Y. Ohyama, T. Suzuki, M. Yamada, K. Makino, H. Ide

Research output: Contribution to journal › Article › peer-review

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Original language	English
Pages (from-to)	87-88
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	44
DOIs	https://doi.org/10.1093/nass/44.1.87
Publication status	Published - 2000
Externally published	Yes

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Medicine(all)

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T1 - Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

AU - Masaoka, A.

AU - Terato, H.

AU - Honsho, A.

AU - Ohyama, Y.

AU - Suzuki, T.

AU - Yamada, M.

AU - Makino, K.

AU - Ide, H.

PY - 2000

Y1 - 2000

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AB - Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

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JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

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Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

Abstract

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