Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

A. Masaoka, H. Terato, A. Honsho, Y. Ohyama, T. Suzuki, M. Yamada, K. Makino, H. Ide

Research output: Contribution to journalArticle

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Original languageEnglish
Pages (from-to)87-88
Number of pages2
JournalNucleic acids symposium series
Issue number44
DOIs
Publication statusPublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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