Preparation and characterization of recombinant golgin tethers

Ayano Satoh, Matthew Beard, Graham Warren

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

Original languageEnglish
Article number26
Pages (from-to)279-296
Number of pages18
JournalMethods in Enzymology
Volume404
DOIs
Publication statusPublished - 2006
Externally publishedYes

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Monomeric GTP-Binding Proteins
Proteins
macrogolgin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Preparation and characterization of recombinant golgin tethers. / Satoh, Ayano; Beard, Matthew; Warren, Graham.

In: Methods in Enzymology, Vol. 404, 26, 2006, p. 279-296.

Research output: Contribution to journal › Article

Satoh, A, Beard, M & Warren, G 2006, 'Preparation and characterization of recombinant golgin tethers', Methods in Enzymology, vol. 404, 26, pp. 279-296. https://doi.org/10.1016/S0076-6879(05)04004-8
Satoh A, Beard M, Warren G. Preparation and characterization of recombinant golgin tethers. Methods in Enzymology. 2006;404:279-296. 26. https://doi.org/10.1016/S0076-6879(05)04004-8
Satoh, Ayano ; Beard, Matthew ; Warren, Graham. / Preparation and characterization of recombinant golgin tethers. In: Methods in Enzymology. 2006 ; Vol. 404. pp. 279-296.
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