Preparation and characterization of recombinant golgin tethers

Ayano Satoh, Matthew Beard, Graham Warren

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

Original languageEnglish
Article number26
Pages (from-to)279-296
Number of pages18
JournalMethods in Enzymology
Volume404
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Monomeric GTP-Binding Proteins
Proteins
macrogolgin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Preparation and characterization of recombinant golgin tethers. / Satoh, Ayano; Beard, Matthew; Warren, Graham.

In: Methods in Enzymology, Vol. 404, 26, 2006, p. 279-296.

Research output: Contribution to journalArticle

Satoh, Ayano ; Beard, Matthew ; Warren, Graham. / Preparation and characterization of recombinant golgin tethers. In: Methods in Enzymology. 2006 ; Vol. 404. pp. 279-296.
@article{15e1059f12294f67a665e246eb887f55,
title = "Preparation and characterization of recombinant golgin tethers",
abstract = "Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.",
author = "Ayano Satoh and Matthew Beard and Graham Warren",
year = "2006",
doi = "10.1016/S0076-6879(05)04004-8",
language = "English",
volume = "404",
pages = "279--296",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Preparation and characterization of recombinant golgin tethers

AU - Satoh, Ayano

AU - Beard, Matthew

AU - Warren, Graham

PY - 2006

Y1 - 2006

N2 - Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

AB - Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

UR - http://www.scopus.com/inward/record.url?scp=30544442204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30544442204&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(05)04004-8

DO - 10.1016/S0076-6879(05)04004-8

M3 - Article

VL - 404

SP - 279

EP - 296

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

M1 - 26

ER -