Abstract
Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.
Original language | English |
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Article number | 26 |
Pages (from-to) | 279-296 |
Number of pages | 18 |
Journal | Methods in Enzymology |
Volume | 404 |
Publication status | Published - Jan 1 2005 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology