Preparation and characterization of recombinant golgin tethers

Ayano Satoh, Matthew Beard, Graham Warren

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

Original languageEnglish
Article number26
Pages (from-to)279-296
Number of pages18
JournalMethods in Enzymology
Volume404
DOIs
Publication statusPublished - 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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