TY - JOUR
T1 - Preparation and characterization of recombinant golgin tethers
AU - Satoh, Ayano
AU - Beard, Matthew
AU - Warren, Graham
N1 - Funding Information:
We thank all members of the Warren, Mellman, and Toomre laboratories for helpful comments and discussions, and Lesa G., Lowe M., Barr F., Nakamura N., Nussbaum R., and Munro S. for generous provision of plasmids. This work was supported by the National Institutes of Health and the Ludwig Institute for Cancer Research. A.S. was supported by the American Heart Association.
PY - 2005
Y1 - 2005
N2 - Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.
AB - Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.
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M3 - Review article
C2 - 16413277
AN - SCOPUS:30544442204
VL - 404
SP - 279
EP - 296
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
M1 - 26
ER -