Preparation and characterization of recombinant golgin tethers

Ayano Satoh; Matthew Beard; Graham Warren

Preparation and characterization of recombinant golgin tethers

Ayano Satoh, Matthew Beard, Graham Warren

Research output: Contribution to journal › Review article › peer-review

Abstract

Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

Original language	English
Article number	26
Pages (from-to)	279-296
Number of pages	18
Journal	Methods in Enzymology
Volume	404
Publication status	Published - Jan 1 2005
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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title = "Preparation and characterization of recombinant golgin tethers",

abstract = "Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.",

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AU - Warren, Graham

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AB - Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

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