Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Isao Date; Yasuyuki Miyoshi; Takeshi Ono; Takashi Imaoka; Tomohisa Furuta; Shoji Asari; Takashi Ohmoto; Hiroo Iwata

doi:10.1016/0963-6897(96)00032-2

Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Isao Date, Yasuyuki Miyoshi, Takeshi Ono, Takashi Imaoka, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto, Hiroo Iwata

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

Original language	English
Pages (from-to)	S17-S19
Journal	Cell Transplantation
Volume	5
Issue number	5 SUPPL. 1
DOIs	https://doi.org/10.1016/0963-6897(96)00032-2
Publication status	Published - 1996

Keywords

Dopamine
Encapsulated cell
Neural transplantation
Parkinson's disease

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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10.1016/0963-6897(96)00032-2

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title = "Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain",

abstract = "Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.",

keywords = "Dopamine, Encapsulated cell, Neural transplantation, Parkinson's disease",

author = "Isao Date and Yasuyuki Miyoshi and Takeshi Ono and Takashi Imaoka and Tomohisa Furuta and Shoji Asari and Takashi Ohmoto and Hiroo Iwata",

note = "Funding Information: Acknowledgments - This work was supported in part by the Fujita Memorial Fund for Medical Research, a Japan Brain Foundation Research Grant to Isao Date and Grants-in-Aid from the Ministry of Education, Science and Culture, Japan, 07671521 to Isao Date and 07457317 to Takashi Ohmoto.",

year = "1996",

doi = "10.1016/0963-6897(96)00032-2",

language = "English",

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AU - Date, Isao

AU - Miyoshi, Yasuyuki

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AU - Imaoka, Takashi

AU - Furuta, Tomohisa

AU - Asari, Shoji

AU - Ohmoto, Takashi

AU - Iwata, Hiroo

N1 - Funding Information: Acknowledgments - This work was supported in part by the Fujita Memorial Fund for Medical Research, a Japan Brain Foundation Research Grant to Isao Date and Grants-in-Aid from the Ministry of Education, Science and Culture, Japan, 07671521 to Isao Date and 07457317 to Takashi Ohmoto.

PY - 1996

Y1 - 1996

N2 - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

AB - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

KW - Dopamine

KW - Encapsulated cell

KW - Neural transplantation

KW - Parkinson's disease

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Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Abstract

Keywords

ASJC Scopus subject areas

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