Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Isao Date, Yasuyuki Miyoshi, Takeshi Ono, Takashi Imaoka, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto, Hiroo Iwata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

Original languageEnglish
JournalCell Transplantation
Volume5
Issue number5 SUPPL. 1
DOIs
Publication statusPublished - Sep 1996

Fingerprint

PC12 Cells
Dopamine
Brain
Polymers
Immunosuppression
Parkinson Disease
Pallidotomy
Styrene
Sulfonic Acids
Cell Transplantation
Tyrosine 3-Monooxygenase
High performance liquid chromatography
Antibodies
Sepharose
Tumors
High Pressure Liquid Chromatography
Acids
Therapeutics
Neoplasms

Keywords

  • Dopamine
  • Encapsulated cell
  • Neural transplantation
  • Parkinson's disease

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain. / Date, Isao; Miyoshi, Yasuyuki; Ono, Takeshi; Imaoka, Takashi; Furuta, Tomohisa; Asari, Shoji; Ohmoto, Takashi; Iwata, Hiroo.

In: Cell Transplantation, Vol. 5, No. 5 SUPPL. 1, 09.1996.

Research output: Contribution to journalArticle

Date, I, Miyoshi, Y, Ono, T, Imaoka, T, Furuta, T, Asari, S, Ohmoto, T & Iwata, H 1996, 'Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain', Cell Transplantation, vol. 5, no. 5 SUPPL. 1. https://doi.org/10.1016/0963-6897(96)00032-2
Date, Isao ; Miyoshi, Yasuyuki ; Ono, Takeshi ; Imaoka, Takashi ; Furuta, Tomohisa ; Asari, Shoji ; Ohmoto, Takashi ; Iwata, Hiroo. / Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain. In: Cell Transplantation. 1996 ; Vol. 5, No. 5 SUPPL. 1.
@article{c4fc9e66331c42d893e0665b0bdeb16f,
title = "Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain",
abstract = "Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.",
keywords = "Dopamine, Encapsulated cell, Neural transplantation, Parkinson's disease",
author = "Isao Date and Yasuyuki Miyoshi and Takeshi Ono and Takashi Imaoka and Tomohisa Furuta and Shoji Asari and Takashi Ohmoto and Hiroo Iwata",
year = "1996",
month = "9",
doi = "10.1016/0963-6897(96)00032-2",
language = "English",
volume = "5",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "5 SUPPL. 1",

}

TY - JOUR

T1 - Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

AU - Date, Isao

AU - Miyoshi, Yasuyuki

AU - Ono, Takeshi

AU - Imaoka, Takashi

AU - Furuta, Tomohisa

AU - Asari, Shoji

AU - Ohmoto, Takashi

AU - Iwata, Hiroo

PY - 1996/9

Y1 - 1996/9

N2 - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

AB - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

KW - Dopamine

KW - Encapsulated cell

KW - Neural transplantation

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=0029773404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029773404&partnerID=8YFLogxK

U2 - 10.1016/0963-6897(96)00032-2

DO - 10.1016/0963-6897(96)00032-2

M3 - Article

C2 - 8889222

AN - SCOPUS:0029773404

VL - 5

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 5 SUPPL. 1

ER -