Abstract
Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.
| Original language | English |
|---|---|
| Journal | Cell Transplantation |
| Volume | 5 |
| Issue number | 5 SUPPL. 1 |
| DOIs | |
| Publication status | Published - Sep 1996 |
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Keywords
- Dopamine
- Encapsulated cell
- Neural transplantation
- Parkinson's disease
ASJC Scopus subject areas
- Cell Biology
- Transplantation
Cite this
Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain. / Date, Isao; Miyoshi, Yasuyuki; Ono, Takeshi; Imaoka, Takashi; Furuta, Tomohisa; Asari, Shoji; Ohmoto, Takashi; Iwata, Hiroo.
In: Cell Transplantation, Vol. 5, No. 5 SUPPL. 1, 09.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain
AU - Date, Isao
AU - Miyoshi, Yasuyuki
AU - Ono, Takeshi
AU - Imaoka, Takashi
AU - Furuta, Tomohisa
AU - Asari, Shoji
AU - Ohmoto, Takashi
AU - Iwata, Hiroo
PY - 1996/9
Y1 - 1996/9
N2 - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.
AB - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.
KW - Dopamine
KW - Encapsulated cell
KW - Neural transplantation
KW - Parkinson's disease
UR - http://www.scopus.com/inward/record.url?scp=0029773404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029773404&partnerID=8YFLogxK
U2 - 10.1016/0963-6897(96)00032-2
DO - 10.1016/0963-6897(96)00032-2
M3 - Article
C2 - 8889222
AN - SCOPUS:0029773404
VL - 5
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 5 SUPPL. 1
ER -