Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Isao Date; Yasuyuki Miyoshi; Takeshi Ono; Takashi Imaoka; Tomohisa Furuta; Shoji Asari; Takashi Ohmoto; Hiroo Iwata

doi:10.1016/0963-6897(96)00032-2

Preliminary report of polymer-encapsulated dopamine-secreting cell grafting into the brain

Isao Date, Yasuyuki Miyoshi, Takeshi Ono, Takashi Imaoka, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto, Hiroo Iwata

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

Original language	English
Pages (from-to)	S17-S19
Journal	Cell Transplantation
Volume	5
Issue number	5 SUPPL. 1
DOIs	https://doi.org/10.1016/0963-6897(96)00032-2
Publication status	Published - Jan 1 1996

Keywords

Dopamine
Encapsulated cell
Neural transplantation
Parkinson's disease

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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abstract = "Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.",

keywords = "Dopamine, Encapsulated cell, Neural transplantation, Parkinson's disease",

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AU - Date, Isao

AU - Miyoshi, Yasuyuki

AU - Ono, Takeshi

AU - Imaoka, Takashi

AU - Furuta, Tomohisa

AU - Asari, Shoji

AU - Ohmoto, Takashi

AU - Iwata, Hiroo

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N2 - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

AB - Polymer-encapsulated dopamine-secreting cell grafting is one of the most promising approaches for the treatment of Parkinson's disease. We microencapsulated dopamine secreting PC12 cells into agarose/poly(styrene sulfonic acid) complex and grafted them into the xenogeneic brain without immunosuppression. Dopamine secretion from the encapsulated cells was confirmed by high-performance liquid chromatography (HPLC) analysis before grafting. A large number of encapsulated PC12 cells survived in the brain 1 mo after transplantation and these cells were immunoreactive to tyrosine hydroxylase (TH) antibody, suggesting that these cells were secreting dopamine into the brain. There was no apparent immunological rejection or tumor formation. We concluded that microencapsulated PC12 cells survive in the xenogeneic brain without immunosuppression, and this grafting procedure is expected to be applied for the treatment of Parkinson's disease in the near future in combination with stereotaxic thalamotomy or pallidotomy.

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