Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells1[W]

Kiichi Kato, Ivan Galis, Shiori Suzuki, Satoshi Araki, Taku Demura, Marie Claire Criqui, Thomas Potuschak, Pascal Genschik, Hiroo Fukuda, Ken Matsuoka, Masaki Ito

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phasespecific enhancers and bind with R1R2R3-Myb transcription factors. Here, we examined the genome-wide effects of NtmybA2 overexpression, one of the R1R2R3-Myb transcription factors in tobacco (Nicotiana tabacum). We used a custom-made 16-K cDNA microarray for comparative transcriptome analysis of transgenic tobacco BY-2 cell lines that overexpress NtmybA2 or its truncated hyperactive form. The microarray was also used to determine the transcript profile during the cell cycle in synchronized cultures of BY-2 cells. Combined microarray data from transgenic lines and synchronized cells revealed that overexpression of the truncated hyperactive form of NtmybA2, but not its full-length form, preferentially up-regulated many G2/M phase-specific genes in BY-2 cells. We determined promoter sequences of several such up-regulated genes and showed that all contain MSA-like motifs in the proximal regions of their promoters. One of the up-regulated genes, NtE2C, encoding for cyclin-specific ubiquitin carrier proteins, contained a single functional MSA-like motif, which specifically controlled the expression of a reporter gene in the G2/M phase in BY-2 cells. Furthermore, a genomic footprint experiment showed that the MSA element in the NtE2C promoter interacted with nuclear proteins in vivo. Therefore, we propose that the transcription of many G2/M phase-specific genes in tobacco is positively regulated by NtmybA2, in most cases through direct binding to the MSA elements.

Original languageEnglish
Pages (from-to)1945-1957
Number of pages13
JournalPlant Physiology
Volume149
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

G2 Phase
Mitosis
Cell Division
mitosis
Tobacco
tobacco
Up-Regulation
Genes
genes
Transcription Factors
Plant Genes
promoter regions
Cyclins
Gene Expression Profiling
Nuclear Proteins
Oligonucleotide Array Sequence Analysis
Reporter Genes
Genetic Promoter Regions
transcription factors
Cell Cycle

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells1[W]. / Kato, Kiichi; Galis, Ivan; Suzuki, Shiori; Araki, Satoshi; Demura, Taku; Criqui, Marie Claire; Potuschak, Thomas; Genschik, Pascal; Fukuda, Hiroo; Matsuoka, Ken; Ito, Masaki.

In: Plant Physiology, Vol. 149, No. 4, 04.2009, p. 1945-1957.

Research output: Contribution to journalArticle

Kato, Kiichi ; Galis, Ivan ; Suzuki, Shiori ; Araki, Satoshi ; Demura, Taku ; Criqui, Marie Claire ; Potuschak, Thomas ; Genschik, Pascal ; Fukuda, Hiroo ; Matsuoka, Ken ; Ito, Masaki. / Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells1[W]. In: Plant Physiology. 2009 ; Vol. 149, No. 4. pp. 1945-1957.
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AU - Criqui, Marie Claire

AU - Potuschak, Thomas

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AU - Fukuda, Hiroo

AU - Matsuoka, Ken

AU - Ito, Masaki

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