Preferential distribution of boron to developing tissues is mediated by the intrinsic protein OSNIP3

Shao Jifeng, Naoki Yamaji, Xin Wei Liu, Kengo Yokosho, Ren Fang Shen, Jian Feng Ma

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Boron is especially required for the growth of meristem and reproductive organs, but the molecular mechanisms underlying the preferential distribution of B to these developing tissues are poorly understood. Here, we show evidence that a member of nodulin 26-like intrinsic protein (NIP), OsNIP3;1, is involved in this preferential distribution in rice (Oryza sativa). OsNIP3;1 was highly expressed in the nodes and its expression was up-regulated by B deficiency, but down-regulated by high B. OsNIP3;1 was polarly localized at the xylem parenchyma cells of enlarged vascular bundles of nodes facing toward the xylem vessels. Furthermore, this protein was rapidly degraded within a few hours in response to high B. Knockout of this gene hardly affected the uptake and root-to-shoot translocation of B, but altered B distribution in different organs in the above-ground parts, decreased distribution of B to the new leaves, and increased distribution to the old leaves. These results indicate that OsNIP3;1 located in the nodes is involved in the preferential distribution of B to the developing tissues by unloading B from the xylem in rice and that it is regulated at both transcriptional and protein level in response to external B level.

Original languageEnglish
Pages (from-to)1739-1750
Number of pages12
JournalPlant Physiology
Volume176
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Xylem
Boron
boron
xylem
nodulins
rice
Gene Knockout Techniques
Proteins
Meristem
proteins
xylem vessels
vascular bundles
gene targeting
meristems
parenchyma (plant tissue)
aerial parts
Blood Vessels
leaves
Oryza sativa
uptake mechanisms

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Preferential distribution of boron to developing tissues is mediated by the intrinsic protein OSNIP3. / Jifeng, Shao; Yamaji, Naoki; Liu, Xin Wei; Yokosho, Kengo; Shen, Ren Fang; Ma, Jian Feng.

In: Plant Physiology, Vol. 176, No. 2, 01.02.2018, p. 1739-1750.

Research output: Contribution to journalArticle

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AU - Ma, Jian Feng

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