Predominance of PCR-ribotypes, 018 (Smz) and 369 (trf) of clostridium difficile in Japan: A potential relationship with other global circulating strains?

Mitsutoshi Senoh, Haru Kato, Tadashi Fukuda, Akiko Niikawa, Yoshiko Hori, Hideharu Hagiya, Yoichiro Ito, Hiroshi Miki, Yoshifumi Abe, Kiyoshi Furuta, Hideki Takeuchi, Hirokazu Tajima, Harumi Tominaga, Hideyuki Satomura, Hideaki Kato, Sayuri Morita, Ai Tanada, Toshinori Hara, Miki Kawada, Yuka SatoMasahiko Takahashi, Akiko Higuchi, Tomoko Nakajima, Yukiko Wakamatsu, Masahiro Toyokawa, Akiko Ueda, Paul Roberts, Fabio Miyajima, Keigo Shibayama

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Global spread and evolutionary links of an epidemic Clostridium difficile strain (PCR-ribotype 027) have been noted in recent decades. However, in Japan, no outbreaks caused by type 027 have been reported to date. A total of 120 C. difficile isolates from patients at 15 hospitals during non-outbreak seasons between 2011 and 2013 as well as 18 and 21 isolates collected from two hospitals in 2010 and 2009, respectively, in outbreak periods in Japan, were examined. Among these 120 isolates, Japan-ribotypes smz and ysmz (subtype variant of smz) were the most predominant (39.2%) followed by Japan-ribotype trf (15.8%). Types smz/ysmz and trf were also concurrently predominant at two hospitals in the outbreak settings. Out of the five binary toxin-positive isolates observed, only one was PCR-ribotype 027 and another PCRribotype 078. Type smz was later found to correspond to PCR-ribotype 018. High rates of resistance against atifloxacin, moxifloxacin, erythromycin and clindamycin were observed in the PCR-ribotype 018 isolates. Interestingly, all trf isolates were toxin A-negative, toxin B-positive, but they did not correspond to PCR-ribotype 017, thus being assigned a new ribotype (PCRribotype 369). In conclusion, PCR-ribotypes 018 (smz) and 369 (trf) were identified as major circulating strains in both outbreak and non-outbreak settings in Japan. Given their epidemiological relevance, molecular investigations are warranted to clarify potential evolutionary links with related strains found elsewhere, such as PCR-ribotypes 018 and 017 from Europe and North America.

Original languageEnglish
Pages (from-to)1226-1236
Number of pages11
JournalJournal of Medical Microbiology
Volume64
Issue number10
DOIs
Publication statusPublished - Oct 2015
Externally publishedYes

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Ribotyping
Clostridium difficile
Japan
Polymerase Chain Reaction
Disease Outbreaks
Clindamycin
Erythromycin
North America

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Predominance of PCR-ribotypes, 018 (Smz) and 369 (trf) of clostridium difficile in Japan : A potential relationship with other global circulating strains? / Senoh, Mitsutoshi; Kato, Haru; Fukuda, Tadashi; Niikawa, Akiko; Hori, Yoshiko; Hagiya, Hideharu; Ito, Yoichiro; Miki, Hiroshi; Abe, Yoshifumi; Furuta, Kiyoshi; Takeuchi, Hideki; Tajima, Hirokazu; Tominaga, Harumi; Satomura, Hideyuki; Kato, Hideaki; Morita, Sayuri; Tanada, Ai; Hara, Toshinori; Kawada, Miki; Sato, Yuka; Takahashi, Masahiko; Higuchi, Akiko; Nakajima, Tomoko; Wakamatsu, Yukiko; Toyokawa, Masahiro; Ueda, Akiko; Roberts, Paul; Miyajima, Fabio; Shibayama, Keigo.

In: Journal of Medical Microbiology, Vol. 64, No. 10, 10.2015, p. 1226-1236.

Research output: Contribution to journalArticle

Senoh, M, Kato, H, Fukuda, T, Niikawa, A, Hori, Y, Hagiya, H, Ito, Y, Miki, H, Abe, Y, Furuta, K, Takeuchi, H, Tajima, H, Tominaga, H, Satomura, H, Kato, H, Morita, S, Tanada, A, Hara, T, Kawada, M, Sato, Y, Takahashi, M, Higuchi, A, Nakajima, T, Wakamatsu, Y, Toyokawa, M, Ueda, A, Roberts, P, Miyajima, F & Shibayama, K 2015, 'Predominance of PCR-ribotypes, 018 (Smz) and 369 (trf) of clostridium difficile in Japan: A potential relationship with other global circulating strains?', Journal of Medical Microbiology, vol. 64, no. 10, pp. 1226-1236. https://doi.org/10.1099/jmm.0.000149
Senoh, Mitsutoshi ; Kato, Haru ; Fukuda, Tadashi ; Niikawa, Akiko ; Hori, Yoshiko ; Hagiya, Hideharu ; Ito, Yoichiro ; Miki, Hiroshi ; Abe, Yoshifumi ; Furuta, Kiyoshi ; Takeuchi, Hideki ; Tajima, Hirokazu ; Tominaga, Harumi ; Satomura, Hideyuki ; Kato, Hideaki ; Morita, Sayuri ; Tanada, Ai ; Hara, Toshinori ; Kawada, Miki ; Sato, Yuka ; Takahashi, Masahiko ; Higuchi, Akiko ; Nakajima, Tomoko ; Wakamatsu, Yukiko ; Toyokawa, Masahiro ; Ueda, Akiko ; Roberts, Paul ; Miyajima, Fabio ; Shibayama, Keigo. / Predominance of PCR-ribotypes, 018 (Smz) and 369 (trf) of clostridium difficile in Japan : A potential relationship with other global circulating strains?. In: Journal of Medical Microbiology. 2015 ; Vol. 64, No. 10. pp. 1226-1236.
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abstract = "Global spread and evolutionary links of an epidemic Clostridium difficile strain (PCR-ribotype 027) have been noted in recent decades. However, in Japan, no outbreaks caused by type 027 have been reported to date. A total of 120 C. difficile isolates from patients at 15 hospitals during non-outbreak seasons between 2011 and 2013 as well as 18 and 21 isolates collected from two hospitals in 2010 and 2009, respectively, in outbreak periods in Japan, were examined. Among these 120 isolates, Japan-ribotypes smz and ysmz (subtype variant of smz) were the most predominant (39.2{\%}) followed by Japan-ribotype trf (15.8{\%}). Types smz/ysmz and trf were also concurrently predominant at two hospitals in the outbreak settings. Out of the five binary toxin-positive isolates observed, only one was PCR-ribotype 027 and another PCRribotype 078. Type smz was later found to correspond to PCR-ribotype 018. High rates of resistance against atifloxacin, moxifloxacin, erythromycin and clindamycin were observed in the PCR-ribotype 018 isolates. Interestingly, all trf isolates were toxin A-negative, toxin B-positive, but they did not correspond to PCR-ribotype 017, thus being assigned a new ribotype (PCRribotype 369). In conclusion, PCR-ribotypes 018 (smz) and 369 (trf) were identified as major circulating strains in both outbreak and non-outbreak settings in Japan. Given their epidemiological relevance, molecular investigations are warranted to clarify potential evolutionary links with related strains found elsewhere, such as PCR-ribotypes 018 and 017 from Europe and North America.",
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AU - Senoh, Mitsutoshi

AU - Kato, Haru

AU - Fukuda, Tadashi

AU - Niikawa, Akiko

AU - Hori, Yoshiko

AU - Hagiya, Hideharu

AU - Ito, Yoichiro

AU - Miki, Hiroshi

AU - Abe, Yoshifumi

AU - Furuta, Kiyoshi

AU - Takeuchi, Hideki

AU - Tajima, Hirokazu

AU - Tominaga, Harumi

AU - Satomura, Hideyuki

AU - Kato, Hideaki

AU - Morita, Sayuri

AU - Tanada, Ai

AU - Hara, Toshinori

AU - Kawada, Miki

AU - Sato, Yuka

AU - Takahashi, Masahiko

AU - Higuchi, Akiko

AU - Nakajima, Tomoko

AU - Wakamatsu, Yukiko

AU - Toyokawa, Masahiro

AU - Ueda, Akiko

AU - Roberts, Paul

AU - Miyajima, Fabio

AU - Shibayama, Keigo

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N2 - Global spread and evolutionary links of an epidemic Clostridium difficile strain (PCR-ribotype 027) have been noted in recent decades. However, in Japan, no outbreaks caused by type 027 have been reported to date. A total of 120 C. difficile isolates from patients at 15 hospitals during non-outbreak seasons between 2011 and 2013 as well as 18 and 21 isolates collected from two hospitals in 2010 and 2009, respectively, in outbreak periods in Japan, were examined. Among these 120 isolates, Japan-ribotypes smz and ysmz (subtype variant of smz) were the most predominant (39.2%) followed by Japan-ribotype trf (15.8%). Types smz/ysmz and trf were also concurrently predominant at two hospitals in the outbreak settings. Out of the five binary toxin-positive isolates observed, only one was PCR-ribotype 027 and another PCRribotype 078. Type smz was later found to correspond to PCR-ribotype 018. High rates of resistance against atifloxacin, moxifloxacin, erythromycin and clindamycin were observed in the PCR-ribotype 018 isolates. Interestingly, all trf isolates were toxin A-negative, toxin B-positive, but they did not correspond to PCR-ribotype 017, thus being assigned a new ribotype (PCRribotype 369). In conclusion, PCR-ribotypes 018 (smz) and 369 (trf) were identified as major circulating strains in both outbreak and non-outbreak settings in Japan. Given their epidemiological relevance, molecular investigations are warranted to clarify potential evolutionary links with related strains found elsewhere, such as PCR-ribotypes 018 and 017 from Europe and North America.

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