Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein

Ikuo Kuroda, Junko Inagaki, Yasusi Yamamoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalPlant Molecular Biology
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1993

Fingerprint

Euglena
Euglena gracilis
Polyproteins
Photosystem II Protein Complex
Chloroplasts
Nuclear Proteins
nuclear-encoded proteins
photosystem II
Light-Harvesting Protein Complexes
Ribulose-Bisphosphate Carboxylase
Oxygenases
Apoproteins
Protein Biosynthesis
chloroplasts
Immunoprecipitation
Carrier Proteins
Proteins
apoproteins
proteins
transport proteins

Keywords

  • chloroplast
  • Euglena gracilis Z
  • oxygen evolution
  • photosystem II
  • precursor protein
  • protein transport

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein. / Kuroda, Ikuo; Inagaki, Junko; Yamamoto, Yasusi.

In: Plant Molecular Biology, Vol. 21, No. 1, 01.1993, p. 171-176.

Research output: Contribution to journalArticle

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