Abstract
Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.
| Original language | English |
|---|---|
| Pages (from-to) | 171-176 |
| Number of pages | 6 |
| Journal | Plant Molecular Biology |
| Volume | 21 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1993 |
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Keywords
- chloroplast
- Euglena gracilis Z
- oxygen evolution
- photosystem II
- precursor protein
- protein transport
ASJC Scopus subject areas
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein. / Kuroda, Ikuo; Inagaki, Junko; Yamamoto, Yasusi.
In: Plant Molecular Biology, Vol. 21, No. 1, 01.1993, p. 171-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein
AU - Kuroda, Ikuo
AU - Inagaki, Junko
AU - Yamamoto, Yasusi
PY - 1993/1
Y1 - 1993/1
N2 - Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.
AB - Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.
KW - chloroplast
KW - Euglena gracilis Z
KW - oxygen evolution
KW - photosystem II
KW - precursor protein
KW - protein transport
UR - http://www.scopus.com/inward/record.url?scp=0027350948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027350948&partnerID=8YFLogxK
U2 - 10.1007/BF00039627
DO - 10.1007/BF00039627
M3 - Article
C2 - 7678760
AN - SCOPUS:0027350948
VL - 21
SP - 171
EP - 176
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 1
ER -