Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein

Ikuo Kuroda; Junko Inagaki; Yasusi Yamamoto

doi:10.1007/BF00039627

Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein

Ikuo Kuroda, Junko Inagaki, Yasusi Yamamoto

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.

Original language	English
Pages (from-to)	171-176
Number of pages	6
Journal	Plant Molecular Biology
Volume	21
Issue number	1
DOIs	https://doi.org/10.1007/BF00039627
Publication status	Published - Jan 1 1993

Keywords

Euglena gracilis Z
chloroplast
oxygen evolution
photosystem II
precursor protein
protein transport

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/BF00039627

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abstract = "Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.",

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AU - Kuroda, Ikuo

AU - Inagaki, Junko

AU - Yamamoto, Yasusi

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