Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

Amy C. Luce, Anupma Sharma, Oliver S.B. Mollere, Thomas K. Wolfgruber, Kiyotaka Nagaki, Jiming Jiang, Gernot G. Presting, R. Kelly Dawe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

Original languageEnglish
Pages (from-to)1057-1061
Number of pages5
JournalGenetics
Volume174
Issue number2
DOIs
Publication statusPublished - Nov 6 2006

ASJC Scopus subject areas

  • Genetics

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    Luce, A. C., Sharma, A., Mollere, O. S. B., Wolfgruber, T. K., Nagaki, K., Jiang, J., Presting, G. G., & Dawe, R. K. (2006). Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction. Genetics, 174(2), 1057-1061. https://doi.org/10.1534/genetics.106.060467