Pre-coating with serum albumin reduces receptor-mediated hepatic disposition of polystyrene nanosphere: Implications for rational design of nanoparticles

Ken Ichi Ogawara, Kentaro Furumoto, Susumu Nagayama, Keiko Minato, Kazutaka Higaki, Toshiya Kai, Toshikiro Kimura

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

We evaluated the in vivo disposition characteristics of polystyrene nanospheres (NS) with the particle size of 50 nm (NS-50) pre-coated with human serum albumin (HSA) after intravenous administration in rats. HSA-coated NS-50 showed much longer blood-circulating property and the hepatic uptake clearance for HSA-coated NS-50 was about 1/5 of that for NS-50. In parallel with the results obtained in the in vivo study, liver perfusion experiments also showed that the hepatic disposition of HSA-coated NS-50 was much less than that of NS-50 in the presence of serum in the perfusate. To unravel the mechanism behind the less affinity of HSA-coated NS-50 to the liver, serum proteins associated on the surface was quantitatively and qualitatively assessed. The results indicated that pre-coated HSA impaired subsequent association of serum proteins onto the surface, suggesting that the association of a given serum protein with opsonic activity might be suppressed by HSA pre-coating. From these findings, pre-coating of nanoparticles with serum albumin could be useful to prevent their rapid clearance by mononuclear phagocyte system in vivo.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalJournal of Controlled Release
Volume100
Issue number3
DOIs
Publication statusPublished - Dec 10 2004

Keywords

  • Hepatic uptake
  • Opsonins
  • Polystyrene nanosphere
  • Receptor-mediated phagocytosis
  • Serum albumin

ASJC Scopus subject areas

  • Pharmaceutical Science

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