TY - JOUR
T1 - Practical aspects of uniform stable isotope labeling of higher plants for heteronuclear NMR-based metabolomics.
AU - Kikuchi, Jun
AU - Hirayama, Takashi
PY - 2007
Y1 - 2007
N2 - Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
AB - Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
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U2 - 10.1007/978-1-59745-244-1_15
DO - 10.1007/978-1-59745-244-1_15
M3 - Article
C2 - 17035691
AN - SCOPUS:34547704068
VL - 358
SP - 273
EP - 286
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -