pp60c-src Expression in Rat Spermatogenesis

H. Nishio, M. Tokuda, T. Itano, Hideki Matsui, Y. Takeuchi, O. Hatase

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src(mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.

Original languageEnglish
Pages (from-to)502-510
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume206
Issue number2
DOIs
Publication statusPublished - Jan 17 1995
Externally publishedYes

Fingerprint

Spermatogenesis
Rats
src-Family Kinases
Testis
Spermatids
Meiosis
Fractionation
Assays
Phosphotransferases
Genes
Monoclonal Antibodies
Staining and Labeling
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Nishio, H., Tokuda, M., Itano, T., Matsui, H., Takeuchi, Y., & Hatase, O. (1995). pp60c-src Expression in Rat Spermatogenesis. Biochemical and Biophysical Research Communications, 206(2), 502-510. https://doi.org/10.1006/bbrc.1995.1072

pp60c-src Expression in Rat Spermatogenesis. / Nishio, H.; Tokuda, M.; Itano, T.; Matsui, Hideki; Takeuchi, Y.; Hatase, O.

In: Biochemical and Biophysical Research Communications, Vol. 206, No. 2, 17.01.1995, p. 502-510.

Research output: Contribution to journalArticle

Nishio, H, Tokuda, M, Itano, T, Matsui, H, Takeuchi, Y & Hatase, O 1995, 'pp60c-src Expression in Rat Spermatogenesis', Biochemical and Biophysical Research Communications, vol. 206, no. 2, pp. 502-510. https://doi.org/10.1006/bbrc.1995.1072
Nishio H, Tokuda M, Itano T, Matsui H, Takeuchi Y, Hatase O. pp60c-src Expression in Rat Spermatogenesis. Biochemical and Biophysical Research Communications. 1995 Jan 17;206(2):502-510. https://doi.org/10.1006/bbrc.1995.1072
Nishio, H. ; Tokuda, M. ; Itano, T. ; Matsui, Hideki ; Takeuchi, Y. ; Hatase, O. / pp60c-src Expression in Rat Spermatogenesis. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 206, No. 2. pp. 502-510.
@article{72ef5aa3b630475999ee5e70877d13ee,
title = "pp60c-src Expression in Rat Spermatogenesis",
abstract = "A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src(mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.",
author = "H. Nishio and M. Tokuda and T. Itano and Hideki Matsui and Y. Takeuchi and O. Hatase",
year = "1995",
month = "1",
day = "17",
doi = "10.1006/bbrc.1995.1072",
language = "English",
volume = "206",
pages = "502--510",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - pp60c-src Expression in Rat Spermatogenesis

AU - Nishio, H.

AU - Tokuda, M.

AU - Itano, T.

AU - Matsui, Hideki

AU - Takeuchi, Y.

AU - Hatase, O.

PY - 1995/1/17

Y1 - 1995/1/17

N2 - A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src(mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.

AB - A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src(mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0028985415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028985415&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1072

DO - 10.1006/bbrc.1995.1072

M3 - Article

C2 - 7530000

AN - SCOPUS:0028985415

VL - 206

SP - 502

EP - 510

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -