Potential use of ultrasound in creating transmyocardial channels

S. Yamamoto, T. Kohmoto, K. Kino, H. Yoshida, S. Sano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Although the mechanisms of the clinical benefits of transmyocardial laser revascularization (TMLR) are considered to be angiogenesis with increased perfusion, denervation and placebo effect, it is unknown whether laser energy is a prerequisite in obtaining these beneficial effects. The present study investigated whether it is possible to create transmyocardial channels and induce angiogenesis by ultrasound. Myocardium was penetrated with an ultrasonically activated surgical blade by advancing the blade tip perpendicularly to the left ventricular free wall of the beating heart of 6 mongrel dogs. The power of ultrasound was set at either the lowest or highest of the system. The animals were killed 30 min (acute; n=3) and 2 weeks (chronic; n=3) after channel creation. Holmium:YAG laser, which is currently used for clinical TMLR, was used to create myocardial channels in 4 other dogs, which were also killed 30 min (n=2) and 2 weeks (n=2) after channel creation. The areas of acute channel core, acute thermal damage and chronic fibrosis were compared between the laser and ultrasound channels by Masson's trichrome stain. Factor VIII and proliferating cell nuclear antigen (PCNA) immunostaining were carried out on the samples obtained from chronic animals. The density of vessels and that of proliferating vascular endothelial cells and vascular smooth muscle cells around the channels were measured. The area of acute core was larger in the lowest and highest outputs of ultrasound than in laser channels (0.78±0.09, 1.0±0.12 vs 0.38±0.04 mm2; p2; p2; p

Original languageEnglish
Pages (from-to)565-571
Number of pages7
JournalJapanese Circulation Journal
Volume65
Issue number6
DOIs
Publication statusPublished - 2001

Fingerprint

Transmyocardial Laser Revascularization
Lasers
Dogs
Placebo Effect
Solid-State Lasers
Factor VIII
Proliferating Cell Nuclear Antigen
Denervation
Vascular Smooth Muscle
Smooth Muscle Myocytes
Myocardium
Fibrosis
Endothelial Cells
Perfusion
Hot Temperature

Keywords

  • Angiogenesis
  • Transmyocardial channels
  • Transmyocardial laser revascularization
  • Ultrasound

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology

Cite this

Yamamoto, S., Kohmoto, T., Kino, K., Yoshida, H., & Sano, S. (2001). Potential use of ultrasound in creating transmyocardial channels. Japanese Circulation Journal, 65(6), 565-571. https://doi.org/10.1253/jcj.65.565

Potential use of ultrasound in creating transmyocardial channels. / Yamamoto, S.; Kohmoto, T.; Kino, K.; Yoshida, H.; Sano, S.

In: Japanese Circulation Journal, Vol. 65, No. 6, 2001, p. 565-571.

Research output: Contribution to journalArticle

Yamamoto, S, Kohmoto, T, Kino, K, Yoshida, H & Sano, S 2001, 'Potential use of ultrasound in creating transmyocardial channels', Japanese Circulation Journal, vol. 65, no. 6, pp. 565-571. https://doi.org/10.1253/jcj.65.565
Yamamoto, S. ; Kohmoto, T. ; Kino, K. ; Yoshida, H. ; Sano, S. / Potential use of ultrasound in creating transmyocardial channels. In: Japanese Circulation Journal. 2001 ; Vol. 65, No. 6. pp. 565-571.
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