Potential role of hCG in apoptosis of human luteinized granulosa cells

Rei Hirata, Takuo Hojo, Masahiro Sano, Nobuyoshi Hayashi, Kiyoshi Okuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The corpus luteum (CL) forms after ovulation and acts as a temporary endocrine gland that produces progesterone (P4), a hormone that is essential for implantation and maintenance of pregnancy in mammals. In pregnant women, human chorionic gonadotropin (hCG) secreted by the conceptus prevents luteolysis. hCG also increases the survival of cultured human luteinized granulosa cells (hLGCs). To clarify the maintenance mechanism of the human CL, we investigated the effects of hCG and P4 receptor antagonists, onapristone (OP) and RU486, on the viability of hLGCs. With the patients’ consent, hLGCs were isolated from follicular aspirates for in vitro fertilization. The cells were cultured with hCG (0.1, 1, 10, 100 IU/ml), OP (10, 25, 50, 100 μM), RU486 (100 μM), P4 (1, 10, 25, 50 μM) or some combination of the four for 24 h. Cell viability was significantly increased by hCG (100 IU/ml) and significantly decreased by OP (100 μM) compared with the control. Cells treated with hCG and OP together were significantly less viable than the control and OP-treated cells. The combined treatment also significantly increased CASP3 activity and cleaved CASP3 protein expression. Furthermore, P4 addition reversed the reduction in cell viability caused by the combination of hCG and OP treatment. The overall findings suggest that hCG cooperates with P4 to increase survival of hLGCs and to induce apoptosis when P4 action supported by hCG is attenuated in the human CL.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalJournal of Reproduction and Development
Volume61
Issue number1
Publication statusPublished - 2015

Fingerprint

human chorionic gonadotropin
granulosa cells
apoptosis
corpus luteum
cell viability
endocrine glands
luteolysis
conceptus
in vitro fertilization
cells
pregnant women
ovulation
antagonists
progesterone
protein synthesis
hormones
viability
pregnancy
mammals
receptors

Keywords

  • Human chorionic gonadotropin
  • Human luteinized granulosa cell
  • Progesterone
  • Survival factor

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Hirata, R., Hojo, T., Sano, M., Hayashi, N., & Okuda, K. (2015). Potential role of hCG in apoptosis of human luteinized granulosa cells. Journal of Reproduction and Development, 61(1), 67-73.

Potential role of hCG in apoptosis of human luteinized granulosa cells. / Hirata, Rei; Hojo, Takuo; Sano, Masahiro; Hayashi, Nobuyoshi; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 61, No. 1, 2015, p. 67-73.

Research output: Contribution to journalArticle

Hirata, R, Hojo, T, Sano, M, Hayashi, N & Okuda, K 2015, 'Potential role of hCG in apoptosis of human luteinized granulosa cells', Journal of Reproduction and Development, vol. 61, no. 1, pp. 67-73.
Hirata, Rei ; Hojo, Takuo ; Sano, Masahiro ; Hayashi, Nobuyoshi ; Okuda, Kiyoshi. / Potential role of hCG in apoptosis of human luteinized granulosa cells. In: Journal of Reproduction and Development. 2015 ; Vol. 61, No. 1. pp. 67-73.
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