Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down

Junko Otsuki, Toshiroh Iwasaki, Yuta Tsuji, Yuya Katada, Haruka Sato, Yukari Tsutsumi, Kanae Hatano, Kohyu Furuhashi, Yukiko Matsumoto, Shoji Kokeguchi, Masahide Shiotani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: To determine whether there are differences in size between the male and female pronuclei immediately before the pronuclear membrane breakdown (PNMBD) and to evaluate whether pronuclear size differences influence normal birth rates. Methods: Time-lapse photography was used to measure the size of each pronucleus, while the outcome of 71 frozen-thawed single blastocyst transfers in patients receiving hormone therapy was analyzed retrospectively. The pronuclear measurements were made 4 hours before the PNMBD, corresponding to 16-20 hours after insemination or intracytoplasmic sperm injection, and immediately before the PNMBD. The differences in the areas between the pronuclei in the zygotes that were associated with the live births were compared with those that were associated with the failed pregnancies. Results: The average difference in the area between the pronuclei 4 hours before and immediately before the PNMBD in the patients with a live birth was significantly smaller than in the patients with a failed birth. In addition, the average area difference in the patients with a successful birth was significantly smaller when the measurements were made immediately before the PNMBD, compared with the measurements 4 hours before the PNMBD. Such differences were not detected among the patients who did not achieve a birth. Conclusion: The birth of healthy babies resulted from zygotes that contained pronuclei of similar size when the measurements were made immediately before the PNMBD. Evaluating the size of each pronucleus immediately before the PNMBD provides an effective indicator of the embryo's potential at an early stage of development.

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalReproductive Medicine and Biology
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 1 2017
Externally publishedYes

Fingerprint

Zygote
Live Birth
Membranes
Parturition
Intracytoplasmic Sperm Injections
Insemination
Birth Rate
Photography
Embryo Transfer
Embryonic Structures
Hormones
Pregnancy

Keywords

  • frozen-thawed blastocyst transfer
  • live birth rate
  • pronuclear membrane breakdown
  • pronuclear size
  • time-lapse observation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down. / Otsuki, Junko; Iwasaki, Toshiroh; Tsuji, Yuta; Katada, Yuya; Sato, Haruka; Tsutsumi, Yukari; Hatano, Kanae; Furuhashi, Kohyu; Matsumoto, Yukiko; Kokeguchi, Shoji; Shiotani, Masahide.

In: Reproductive Medicine and Biology, Vol. 16, No. 2, 01.04.2017, p. 200-205.

Research output: Contribution to journalArticle

Otsuki, J, Iwasaki, T, Tsuji, Y, Katada, Y, Sato, H, Tsutsumi, Y, Hatano, K, Furuhashi, K, Matsumoto, Y, Kokeguchi, S & Shiotani, M 2017, 'Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down', Reproductive Medicine and Biology, vol. 16, no. 2, pp. 200-205. https://doi.org/10.1002/rmb2.12032
Otsuki, Junko ; Iwasaki, Toshiroh ; Tsuji, Yuta ; Katada, Yuya ; Sato, Haruka ; Tsutsumi, Yukari ; Hatano, Kanae ; Furuhashi, Kohyu ; Matsumoto, Yukiko ; Kokeguchi, Shoji ; Shiotani, Masahide. / Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down. In: Reproductive Medicine and Biology. 2017 ; Vol. 16, No. 2. pp. 200-205.
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AU - Katada, Yuya

AU - Sato, Haruka

AU - Tsutsumi, Yukari

AU - Hatano, Kanae

AU - Furuhashi, Kohyu

AU - Matsumoto, Yukiko

AU - Kokeguchi, Shoji

AU - Shiotani, Masahide

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AB - Aim: To determine whether there are differences in size between the male and female pronuclei immediately before the pronuclear membrane breakdown (PNMBD) and to evaluate whether pronuclear size differences influence normal birth rates. Methods: Time-lapse photography was used to measure the size of each pronucleus, while the outcome of 71 frozen-thawed single blastocyst transfers in patients receiving hormone therapy was analyzed retrospectively. The pronuclear measurements were made 4 hours before the PNMBD, corresponding to 16-20 hours after insemination or intracytoplasmic sperm injection, and immediately before the PNMBD. The differences in the areas between the pronuclei in the zygotes that were associated with the live births were compared with those that were associated with the failed pregnancies. Results: The average difference in the area between the pronuclei 4 hours before and immediately before the PNMBD in the patients with a live birth was significantly smaller than in the patients with a failed birth. In addition, the average area difference in the patients with a successful birth was significantly smaller when the measurements were made immediately before the PNMBD, compared with the measurements 4 hours before the PNMBD. Such differences were not detected among the patients who did not achieve a birth. Conclusion: The birth of healthy babies resulted from zygotes that contained pronuclei of similar size when the measurements were made immediately before the PNMBD. Evaluating the size of each pronucleus immediately before the PNMBD provides an effective indicator of the embryo's potential at an early stage of development.

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