Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression

R. Nomura, Shuhei Naka, H. Nemoto, M. Otsugu, S. Nakamura, T. Ooshima, K. Nakano

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.

Original languageEnglish
Pages (from-to)1627-1634
Number of pages8
JournalArchives of Oral Biology
Volume58
Issue number11
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Streptococcus mutans
Endocarditis
Virulence
Carrier Proteins
Collagen
Antigens
Proteins
Human Umbilical Vein Endothelial Cells
Dental Caries
Mouth

Keywords

  • Classification
  • Collagen-binding protein
  • Infective endocarditis
  • Protein antigen (PA)
  • Streptococcus mutans

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression. / Nomura, R.; Naka, Shuhei; Nemoto, H.; Otsugu, M.; Nakamura, S.; Ooshima, T.; Nakano, K.

In: Archives of Oral Biology, Vol. 58, No. 11, 2013, p. 1627-1634.

Research output: Contribution to journalArticle

Nomura, R. ; Naka, Shuhei ; Nemoto, H. ; Otsugu, M. ; Nakamura, S. ; Ooshima, T. ; Nakano, K. / Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression. In: Archives of Oral Biology. 2013 ; Vol. 58, No. 11. pp. 1627-1634.
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abstract = "Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10{\%} and ∼3{\%} in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.",
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AU - Nomura, R.

AU - Naka, Shuhei

AU - Nemoto, H.

AU - Otsugu, M.

AU - Nakamura, S.

AU - Ooshima, T.

AU - Nakano, K.

PY - 2013

Y1 - 2013

N2 - Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.

AB - Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.

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KW - Protein antigen (PA)

KW - Streptococcus mutans

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