Lysosomes, isolated from various organs, exhibited an acidic interior ≡ when incubated in a buffer at neutral pH. K+-induced proton efflux was observed in spleen lysosomes, but not in liver or kidney lysosomes. The initial velocity of the proton efflux showed saturation kinetics with Km value of about 15 mM K+. Rb+ and Cs+ have an effect similar to K+, while Na+, Li+ or divalent cations have little or no effect. The properties of the K+ induced proton efflux correlated with the K+-induced depolarization of the lysosomes, suggesting the presence of K+-transport system(s) in lysosomal membranes.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Oct 14 1988|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology