Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation

Yoshiki Mukudai, Satoshi Kubota, Harumi Kawaki, Seiji Kondo, Takanori Eguchi, Kumi Sumiyoshi, Toshihiro Ohgawara, Tsuyoshi Shimo, Masaharu Takigawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

CCN2/CTGF is a multifunctional factor that plays a crucial role in the growth and differentiation of chondrocytes. The chicken ccn2 gene is regulated not only at the transcriptional level but also by the interaction between a posttranscriptional element in the 3′ untranslated region (3′-UTR) and a cofactor. In the present study, we identified a nucleophosmin (NPM) (also called B23) as this cofactor. Binding of NPM to the element was confirmed, and subsequent analysis revealed a significant correlation between the decrease in cytosolic NPM and the increased stability of the ccn2 mRNA during chondrocyte differentiation in vivo. Furthermore, recombinant chicken NPM enhanced the degradation of chimeric RNAs containing the posttranscriptional cis elements in a chicken embryonic fibroblast extract in vitro. It is noteworthy that the RNA destabilization effect by NPM was far more prominent in the cytosolic extract of chondrocytes than in that of fibroblasts, representing a chondrocyte-specific action of NPM. Stimulation by growth factors to promote differentiation changed the subcellular distribution of NPM in chondrocytes, which followed the expected patterns from the resultant change in the ccn2 mRNA stability. Therefore, the present study reveals a novel aspect of NPM as a key player in the posttranscriptional regulation of ccn2 mRNA during the differentiation of chondrocytes.

Original languageEnglish
Pages (from-to)6134-6147
Number of pages14
JournalMolecular and Cellular Biology
Volume28
Issue number19
DOIs
Publication statusPublished - Oct 2008

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Chondrocytes
Chickens
Gene Expression
RNA Stability
Fibroblasts
nucleophosmin
3' Untranslated Regions
Intercellular Signaling Peptides and Proteins
RNA
Messenger RNA
Growth
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation. / Mukudai, Yoshiki; Kubota, Satoshi; Kawaki, Harumi; Kondo, Seiji; Eguchi, Takanori; Sumiyoshi, Kumi; Ohgawara, Toshihiro; Shimo, Tsuyoshi; Takigawa, Masaharu.

In: Molecular and Cellular Biology, Vol. 28, No. 19, 10.2008, p. 6134-6147.

Research output: Contribution to journalArticle

Mukudai, Yoshiki ; Kubota, Satoshi ; Kawaki, Harumi ; Kondo, Seiji ; Eguchi, Takanori ; Sumiyoshi, Kumi ; Ohgawara, Toshihiro ; Shimo, Tsuyoshi ; Takigawa, Masaharu. / Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 19. pp. 6134-6147.
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