Postnatal development of inhibitory synaptic transmission to superior salivatory neurons in rats

Yoshihiro Mitoh, Makoto Funahashi, Masako Fujita, Motoi Kobashi, Ryuji Matsuo

Research output: Contribution to journalArticle

Abstract

The primary parasympathetic center of the submandibular and sublingual salivary glands is the superior salivatory (SS) nucleus, and its neurons receive excitatory (glutamatergic) and inhibitory (GABAergic and glycinergic) synaptic transmissions in rats. In the present study, we focused on the postnatal development of inhibitory transmission to SS neurons. Gramicidin-perforated whole-cell patch-clamp recordings were performed in rat brainstem slices on postnatal day 2 (P2)-P14. Developmental changes in the intracellular Cl - concentration ([Cl-]in) were examined based on the reversal potentials of total inhibitory postsynaptic currents (GABAergic plus glycinergic), which were evoked by electrical stimulation near the recording neuron. The [Cl-]in in the P8-P14 groupwas significantly lower than in the P2-P7 group. The effect of GABA application at the resting potentials changed from depolarization to hyperpolarization around P8, suggesting that SS neurons acquired mature inhibitory systems around P8. The period at which GABA responses change from excitatory to inhibitory in SS neurons was discussed compared with those of the forebrain, brainstem, and spinal neurons.

Original languageEnglish
Pages (from-to)270-272
Number of pages3
JournalJournal of Medical Investigation
Volume56
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2009

Keywords

  • Development
  • GABAergic
  • Glycinergic
  • Gramicidin-perforated patch clamp
  • Superior salivatory neurons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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