Postnatal changes in the expression of genes for cryptdins 1-6 and the role of luminal bacteria in cryptdin gene expression in mouse small intestine

Ryo Inoue, Takeshi Tsuruta, Iyori Nojima, Keizo Nakayama, Takamitsu Tsukahara, Takaji Yajima

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Although there have been many fascinating studies on cryptdins, the information for each cryptdin isoform was not completely provided. In this study, the postnatal changes in the gene expression of cryptdin 1-6 were evaluated, and the patterns of change were compared between conventional and germ-free mice. Two patterns of postnatal change were observed: gene expression of cryptdins 1, 3 and 6 increased gradually, and that of cryptdins 2 and 5 increased rapidly. Gene expression of cryptdin 4 increased gradually in the ileum but rapidly in the jejunum. Conventional mice showed significantly higher gene expression for all isoforms than germ-free mice. Interestingly, the difference in the gene expression for cryptdin 2, 4 and 5 between the jejunum and ileum seemed to be increased by the presence of the luminal bacteria. The results indicate that cryptdin isoforms develop differently depending on the isoform type, and that the gene expression of all cryptdin isoforms was affected by the presence of the luminal bacteria.

Original languageEnglish
Pages (from-to)407-416
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume52
Issue number3
DOIs
Publication statusPublished - Apr 1 2008
Externally publishedYes

Keywords

  • Cryptdin
  • Germ-free
  • Intestinal bacteria
  • Postnatal development

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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