TY - JOUR
T1 - Postischemic expression of P-selectin immunoreactivity in rat brain
AU - Suzuki, H.
AU - Abe, K.
AU - Tojo, S.
AU - Morooka, S.
AU - Kimura, K.
AU - Mizugaki, M.
AU - Itoyama, Y.
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (I. Kanazawa) 07264204 and 08256204, and Grant-in-Aid for Scientific Research (B) 07457152 and (C) 06807055 from the Ministry of Education, Science and Culture of Japan, and by a grant (K. Tashiro) from the Ministry of Health and Welfare of Japan.
PY - 1997/6/13
Y1 - 1997/6/13
N2 - Change of immunoreactive P-selectin was examined in rat brain after transient middle cerebral artery (MCA) occlusion (O) with anti-P-selectin monoclonal antibody using brain samples of sham control and after ischemia. Temporal, spatial, and cellular changes of immunohistochemical expressions of P-selectin were evaluated with rat brain sections at 2 and 8 h, 1, 3, and 7 days of reperfusion after 1 h of MCAO. Western blot showed a single band at molecular weight of 140 kDa for P-selectin after ischemia. P-selectin immunoreactivity was not normally present in rat brain sections. However, it was expressed mainly in the post-capillary venules of the cerebral cortex and caudate in the MCA territory with a peak at 8 h-1 day. The expression was diminished by 3 days of reperfusion. The present results indicate that P-selectin was expressed from an earlier stage of reperfusion in post-capillary venules, and the expression became maximum at the same time both in the cerebral cortex and caudate.
AB - Change of immunoreactive P-selectin was examined in rat brain after transient middle cerebral artery (MCA) occlusion (O) with anti-P-selectin monoclonal antibody using brain samples of sham control and after ischemia. Temporal, spatial, and cellular changes of immunohistochemical expressions of P-selectin were evaluated with rat brain sections at 2 and 8 h, 1, 3, and 7 days of reperfusion after 1 h of MCAO. Western blot showed a single band at molecular weight of 140 kDa for P-selectin after ischemia. P-selectin immunoreactivity was not normally present in rat brain sections. However, it was expressed mainly in the post-capillary venules of the cerebral cortex and caudate in the MCA territory with a peak at 8 h-1 day. The expression was diminished by 3 days of reperfusion. The present results indicate that P-selectin was expressed from an earlier stage of reperfusion in post-capillary venules, and the expression became maximum at the same time both in the cerebral cortex and caudate.
KW - Cerebral ischemia
KW - Middle cerebral artery
KW - P-selectin
KW - Rat
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U2 - 10.1016/S0304-3940(97)00385-6
DO - 10.1016/S0304-3940(97)00385-6
M3 - Article
C2 - 9218630
AN - SCOPUS:0030904614
SN - 0304-3940
VL - 228
SP - 151
EP - 154
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -