Postischemic expression of P-selectin immunoreactivity in rat brain

H. Suzuki, K. Abe, S. Tojo, S. Morooka, K. Kimura, M. Mizugaki, Y. Itoyama

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Change of immunoreactive P-selectin was examined in rat brain after transient middle cerebral artery (MCA) occlusion (O) with anti-P-selectin monoclonal antibody using brain samples of sham control and after ischemia. Temporal, spatial, and cellular changes of immunohistochemical expressions of P-selectin were evaluated with rat brain sections at 2 and 8 h, 1, 3, and 7 days of reperfusion after 1 h of MCAO. Western blot showed a single band at molecular weight of 140 kDa for P-selectin after ischemia. P-selectin immunoreactivity was not normally present in rat brain sections. However, it was expressed mainly in the post-capillary venules of the cerebral cortex and caudate in the MCA territory with a peak at 8 h-1 day. The expression was diminished by 3 days of reperfusion. The present results indicate that P-selectin was expressed from an earlier stage of reperfusion in post-capillary venules, and the expression became maximum at the same time both in the cerebral cortex and caudate.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalNeuroscience Letters
Volume228
Issue number3
DOIs
Publication statusPublished - Jun 13 1997
Externally publishedYes

Keywords

  • Cerebral ischemia
  • Middle cerebral artery
  • P-selectin
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

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