Post-translational regulation of CND41 protease activity in senescent tobacco leaves

Yusuke Kato, Yumiko Yamamoto, Shinya Murakami, Fumihiko Sato

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The degradation of chloroplast proteins is an important occurrence in the mobilization of nutrients from senescing leaves to reproductive organs during senescence. Recently, we proved that tobacco CND41 protease is involved in Rubisco degradation and the translocation of nitrogen during senescence. In this study, we show the post-translational regulation of CND41 protease. Using very specific antibodies that were prepared against CND41-specific peptide (anti-Val 186 to Ser 206), immunoblot analysis clearly indicated a change in the accumulation and processing of CND41 during the maturation of leaves in whole plants. The developmental modification of CND41 was also observed in transgenic tobacco with constitutive expression of CND41 under cauliflower mosaic virus 35S promoter. Further studies of seedlings under senescence induced by combined treatment with nitrogen-starvation and high sucrose confirmed that the processing of CND41 was important for protease activity and senescence. A possible mechanism for the regulation of CND41 activity is discussed.

Original languageEnglish
Pages (from-to)643-651
Number of pages9
JournalPlanta
Volume222
Issue number4
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Fingerprint

Tobacco
Peptide Hydrolases
tobacco
proteinases
leaves
Nitrogen
degradation
Cauliflower mosaic virus
nitrogen
Caulimovirus
ribulose-bisphosphate carboxylase
Chloroplast Proteins
Ribulose-Bisphosphate Carboxylase
starvation
chloroplasts
Starvation
Seedlings
promoter regions
genetically modified organisms
peptides

Keywords

  • Aspartic protease
  • Chloroplast
  • Nitrogen-depletion
  • Post-translational regulation
  • Rubisco
  • Senescence

ASJC Scopus subject areas

  • Plant Science

Cite this

Post-translational regulation of CND41 protease activity in senescent tobacco leaves. / Kato, Yusuke; Yamamoto, Yumiko; Murakami, Shinya; Sato, Fumihiko.

In: Planta, Vol. 222, No. 4, 11.2005, p. 643-651.

Research output: Contribution to journalArticle

Kato, Yusuke ; Yamamoto, Yumiko ; Murakami, Shinya ; Sato, Fumihiko. / Post-translational regulation of CND41 protease activity in senescent tobacco leaves. In: Planta. 2005 ; Vol. 222, No. 4. pp. 643-651.
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