Post-translational modification of flagellin determines the specificity of HR induction

Fumiko Taguchi, Rena Shimizu, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Flagellin, a constituent of the flagellar filament, is a potent elicitor of hypersensitive cell death in plant cells. Flagellins of Pseudomonas syringae pvs. glycinea and tomato induce hypersensitive cell death in their non-host tobacco plants, whereas those of P. syringae pv. tabaci do not remarkably induce it in its host tobacco plants. However, the deduced amino acid sequences of flagellins from pvs. tabaci and glycinea are identical, indicating that post-translational modification of flagellins plays an important role in determining hypersensitive reaction (HR)-inducibility. To investigate genetically the role of modification of flagellin in HR-induction, biological and phytopathological phenotypes of a flagella-defective ΔfliC mutant and ΔfliC mutants complemented by the introduction of the flagellin gene (fliC) from different pathovars of P. syringae were investigated. The ΔfliC mutant of pv. tabaci lost flagella, motility, the ability to induce HR cell death in non-host tomato cells and virulence toward host tobacco plants, whereas all pv. tabaci complemented by the introduction of the fliC gene of pvs. tabaci, glycinea or tomato recovered all the abilities that the ΔfliC mutant had lost. These results indicate that post-translational modification of flagellins is strongly correlated with the ability to cause HR cell death.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalPlant and Cell Physiology
Volume44
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

Fingerprint

Flagellin
flagellin
post-translational modification
hypersensitive response
Post Translational Protein Processing
Pseudomonas syringae
cell death
Cell Death
Lycopersicon esculentum
Tobacco
mutants
tobacco
Flagella
tomatoes
flagellum
Pseudomonas syringae pv. tabaci
Plant Cells
Genes
Virulence
Amino Acid Sequence

Keywords

  • Complementation
  • Elicitor
  • Flagellin
  • fliC
  • HR
  • Post-translational modification

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Post-translational modification of flagellin determines the specificity of HR induction. / Taguchi, Fumiko; Shimizu, Rena; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Plant and Cell Physiology, Vol. 44, No. 3, 01.03.2003, p. 342-349.

Research output: Contribution to journalArticle

Taguchi, Fumiko ; Shimizu, Rena ; Inagaki, Yoshishige ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki. / Post-translational modification of flagellin determines the specificity of HR induction. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 3. pp. 342-349.
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