Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes

Koji Ishikawa, Koji Makanae, Shintaro Iwasaki, Nicholas T. Ingolia, Hisao Moriya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Understanding buffering mechanisms for various perturbations is essential for understanding robustness in cellular systems. Protein-level dosage compensation, which arises when changes in gene copy number do not translate linearly into protein level, is one mechanism for buffering against genetic perturbations. Here, we present an approach to identify genes with dosage compensation by increasing the copy number of individual genes using the genetic tug-of-war technique. Our screen of chromosome I suggests that dosage-compensated genes constitute approximately 10% of the genome and consist predominantly of subunits of multi-protein complexes. Importantly, because subunit levels are regulated in a stoichiometry-dependent manner, dosage compensation plays a crucial role in maintaining subunit stoichiometries. Indeed, we observed changes in the levels of a complex when its subunit stoichiometries were perturbed. We further analyzed compensation mechanisms using a proteasome-defective mutant as well as ribosome profiling, which provided strong evidence for compensation by ubiquitin-dependent degradation but not reduced translational efficiency. Thus, our study provides a systematic understanding of dosage compensation and highlights that this post-translational regulation is a critical aspect of robustness in cellular systems.

Original languageEnglish
Article numbere1006554
JournalPLoS Genetics
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Gene Dosage
stoichiometry
Buffers
buffers
perturbation
gene dosage
protein
dosage
Proteins
proteins
gene
Protein Subunits
Proteasome Endopeptidase Complex
Ubiquitin
multiprotein complexes
buffering
Ribosomes
proteasome endopeptidase complex
ubiquitin
Chromosomes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes. / Ishikawa, Koji; Makanae, Koji; Iwasaki, Shintaro; Ingolia, Nicholas T.; Moriya, Hisao.

In: PLoS Genetics, Vol. 13, No. 1, e1006554, 01.01.2017.

Research output: Contribution to journalArticle

Ishikawa, Koji ; Makanae, Koji ; Iwasaki, Shintaro ; Ingolia, Nicholas T. ; Moriya, Hisao. / Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes. In: PLoS Genetics. 2017 ; Vol. 13, No. 1.
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