Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas

J. D. Rochaix, K. Perron, D. Dauvillée, F. Laroche, Y. Takahashi, M. Goldschmidt-Clermont

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Assembly of the PSI (photosystem I) complex in eukaryotic photosynthetic organisms depends on the concerted interactions of the nuclear and chloroplast genetic systems. We have identified several nucleus-encoded factors of Chlamydomonas reinhardtii that are specifically required for the synthesis of the two large chloroplast-encoded reaction-centre polypeptides, PsaA and PsaB, of photosystem I and that function at plastid post-transcriptional steps. Raa1, Raa2 and Raa3 are required for the splicing of the three discontinuous psaA precursor transcripts; they are part of large RNA-protein complexes that are reminiscent of spliceosomal particles. Tab1 and Tab2 are involved in the initiation of translation of the psaB mRNA and are localized in the membrane and stromal phases of the chloroplast, where they are associated with high-molecular-mass complexes. Moreover, two chloroplast-encoded proteins, Ycf3 and Ycf4, are required for the primary steps of assembling the photosystem I subunits into a functional complex.

    Original languageEnglish
    Pages (from-to)567-570
    Number of pages4
    JournalBiochemical Society Transactions
    Volume32
    Issue number4
    DOIs
    Publication statusPublished - Aug 1 2004

    Keywords

    • Chlamydomonas
    • Chloroplast
    • Photosynthetic mutant
    • Photosystem I
    • Trans-splicing
    • Translation

    ASJC Scopus subject areas

    • Biochemistry

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