Post-mortem tissue-type plasminogen activator preserves graft function of hearts harvested from non-pre-treated non-heart-beating donors

Masanori Hirota, Kozo Ishino, Takeo Tedoriya, Shunji Sano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Intracoronary microthrombi may cause primary graft failure of hearts harvested from non-pre-treated non-heart-beating donors (NHBDs). We examined the extent of functional recovery to compare the protective effects of post-mortem tissue-type plasminogen activator (t-PA) and heparin pre-treatment. Methods: Heparin pre-treatment was systemically administered before hypoxic cardiac arrest in 6 mongrel dogs (Group A). No pre-treatments, including heparin, were administered in 8 dogs (Group B). After 60 minutes of ischemia, intracoronary microthrombi were flushed by retrograde blood cardioplegia with t-PA. After 120 minutes of controlled reperfusion, pre-load was increased for ejection against an after-load of 80 mm Hg. Pressure-volume loops were recorded to obtain the end-systolic pressure-volume relationship (ESPVR) and end-diastolic pressure-volume relationship (EDPVR). Stroke volume at a given pre-load was calculated from averaged ESPVR, EDPVR, and after-load identical to the averaged baseline value. The Frank-Starling relationship was obtained, and cardiac status was classified using the Forrester hemodynamic sub-set. Results: There were no significant differences between Group A and Group B in post-resuscitated end-systolic elastance (3.1 ± 0.7 vs 3.0 ± 0.8 mm Hg/ml), time constant of isovolumic relaxation (40 ± 7 vs 40 ± 6 msec), LV max +dP/dt (1133 ± 131 vs 1090 ± 105 mm Hg/s), and LV max dP/dt (732 ± 131 vs 752 ± 122 mm Hg/s). The post-resuscitated cardiac index was decreased to about 50%, and cardiac status was classified as Forrester III or IV sub-set. Conclusions: Post-mortem t-PA preserves graft function of hearts harvested from non-pre-treated NHBDs. This pharmaceutical intervention may be an alternative to heparin pre-treatment, which could increase the number of cardiac allografts harvested from potential non-pre-treated NHBDs.

Original languageEnglish
Pages (from-to)888-893
Number of pages6
JournalJournal of Heart and Lung Transplantation
Volume29
Issue number8
DOIs
Publication statusPublished - 2010

Fingerprint

Tissue Plasminogen Activator
Heparin
Blood Pressure
Transplants
Plasminogen Activators
Dogs
Starlings
Induced Heart Arrest
Heart Arrest
Stroke Volume
Reperfusion
Allografts
Ischemia
Heart Failure
Hemodynamics
Pressure
Pharmaceutical Preparations

Keywords

  • heart transplantation
  • non-heart-beating donor
  • pre-treatment
  • tissue-type plasminogen activator

ASJC Scopus subject areas

  • Transplantation
  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Post-mortem tissue-type plasminogen activator preserves graft function of hearts harvested from non-pre-treated non-heart-beating donors. / Hirota, Masanori; Ishino, Kozo; Tedoriya, Takeo; Sano, Shunji.

In: Journal of Heart and Lung Transplantation, Vol. 29, No. 8, 2010, p. 888-893.

Research output: Contribution to journalArticle

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abstract = "Background: Intracoronary microthrombi may cause primary graft failure of hearts harvested from non-pre-treated non-heart-beating donors (NHBDs). We examined the extent of functional recovery to compare the protective effects of post-mortem tissue-type plasminogen activator (t-PA) and heparin pre-treatment. Methods: Heparin pre-treatment was systemically administered before hypoxic cardiac arrest in 6 mongrel dogs (Group A). No pre-treatments, including heparin, were administered in 8 dogs (Group B). After 60 minutes of ischemia, intracoronary microthrombi were flushed by retrograde blood cardioplegia with t-PA. After 120 minutes of controlled reperfusion, pre-load was increased for ejection against an after-load of 80 mm Hg. Pressure-volume loops were recorded to obtain the end-systolic pressure-volume relationship (ESPVR) and end-diastolic pressure-volume relationship (EDPVR). Stroke volume at a given pre-load was calculated from averaged ESPVR, EDPVR, and after-load identical to the averaged baseline value. The Frank-Starling relationship was obtained, and cardiac status was classified using the Forrester hemodynamic sub-set. Results: There were no significant differences between Group A and Group B in post-resuscitated end-systolic elastance (3.1 ± 0.7 vs 3.0 ± 0.8 mm Hg/ml), time constant of isovolumic relaxation (40 ± 7 vs 40 ± 6 msec), LV max +dP/dt (1133 ± 131 vs 1090 ± 105 mm Hg/s), and LV max dP/dt (732 ± 131 vs 752 ± 122 mm Hg/s). The post-resuscitated cardiac index was decreased to about 50{\%}, and cardiac status was classified as Forrester III or IV sub-set. Conclusions: Post-mortem t-PA preserves graft function of hearts harvested from non-pre-treated NHBDs. This pharmaceutical intervention may be an alternative to heparin pre-treatment, which could increase the number of cardiac allografts harvested from potential non-pre-treated NHBDs.",
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AU - Tedoriya, Takeo

AU - Sano, Shunji

PY - 2010

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N2 - Background: Intracoronary microthrombi may cause primary graft failure of hearts harvested from non-pre-treated non-heart-beating donors (NHBDs). We examined the extent of functional recovery to compare the protective effects of post-mortem tissue-type plasminogen activator (t-PA) and heparin pre-treatment. Methods: Heparin pre-treatment was systemically administered before hypoxic cardiac arrest in 6 mongrel dogs (Group A). No pre-treatments, including heparin, were administered in 8 dogs (Group B). After 60 minutes of ischemia, intracoronary microthrombi were flushed by retrograde blood cardioplegia with t-PA. After 120 minutes of controlled reperfusion, pre-load was increased for ejection against an after-load of 80 mm Hg. Pressure-volume loops were recorded to obtain the end-systolic pressure-volume relationship (ESPVR) and end-diastolic pressure-volume relationship (EDPVR). Stroke volume at a given pre-load was calculated from averaged ESPVR, EDPVR, and after-load identical to the averaged baseline value. The Frank-Starling relationship was obtained, and cardiac status was classified using the Forrester hemodynamic sub-set. Results: There were no significant differences between Group A and Group B in post-resuscitated end-systolic elastance (3.1 ± 0.7 vs 3.0 ± 0.8 mm Hg/ml), time constant of isovolumic relaxation (40 ± 7 vs 40 ± 6 msec), LV max +dP/dt (1133 ± 131 vs 1090 ± 105 mm Hg/s), and LV max dP/dt (732 ± 131 vs 752 ± 122 mm Hg/s). The post-resuscitated cardiac index was decreased to about 50%, and cardiac status was classified as Forrester III or IV sub-set. Conclusions: Post-mortem t-PA preserves graft function of hearts harvested from non-pre-treated NHBDs. This pharmaceutical intervention may be an alternative to heparin pre-treatment, which could increase the number of cardiac allografts harvested from potential non-pre-treated NHBDs.

AB - Background: Intracoronary microthrombi may cause primary graft failure of hearts harvested from non-pre-treated non-heart-beating donors (NHBDs). We examined the extent of functional recovery to compare the protective effects of post-mortem tissue-type plasminogen activator (t-PA) and heparin pre-treatment. Methods: Heparin pre-treatment was systemically administered before hypoxic cardiac arrest in 6 mongrel dogs (Group A). No pre-treatments, including heparin, were administered in 8 dogs (Group B). After 60 minutes of ischemia, intracoronary microthrombi were flushed by retrograde blood cardioplegia with t-PA. After 120 minutes of controlled reperfusion, pre-load was increased for ejection against an after-load of 80 mm Hg. Pressure-volume loops were recorded to obtain the end-systolic pressure-volume relationship (ESPVR) and end-diastolic pressure-volume relationship (EDPVR). Stroke volume at a given pre-load was calculated from averaged ESPVR, EDPVR, and after-load identical to the averaged baseline value. The Frank-Starling relationship was obtained, and cardiac status was classified using the Forrester hemodynamic sub-set. Results: There were no significant differences between Group A and Group B in post-resuscitated end-systolic elastance (3.1 ± 0.7 vs 3.0 ± 0.8 mm Hg/ml), time constant of isovolumic relaxation (40 ± 7 vs 40 ± 6 msec), LV max +dP/dt (1133 ± 131 vs 1090 ± 105 mm Hg/s), and LV max dP/dt (732 ± 131 vs 752 ± 122 mm Hg/s). The post-resuscitated cardiac index was decreased to about 50%, and cardiac status was classified as Forrester III or IV sub-set. Conclusions: Post-mortem t-PA preserves graft function of hearts harvested from non-pre-treated NHBDs. This pharmaceutical intervention may be an alternative to heparin pre-treatment, which could increase the number of cardiac allografts harvested from potential non-pre-treated NHBDs.

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