Abstract
The adult cricket Gryllus bimaculatus has a central clock in the optic lobe that regulates overt activity rhythms and secondary oscillators in the tissue outside the optic lobe. Here we investigated properties of the rhythmic expression of clock genes in the optic lobe and extra-optic lobe tissues in nymphs, and compared them with those of adults. In the optic lobe, mRNA of the clock genes period (per), timeless (tim), cycle (cyc) and Clock (Clk) were expressed in patterns similar to those in adult profiles, but at significantly lower levels. Among the extra-optic lobe tissues, the brain and TAG showed a rhythmic expression of per and tim, the mid-gut only in tim, and the anterior-stomach in none of the genes studied. The mRNA levels of clock genes were again significantly lower than those in adults. Unlike in adults, the brain and mid-gut lost their rhythms of clock gene expression in DD, and when the optic lobes were bilaterally removed. These results suggest that the rhythms outside the optic lobe are weak in nymphs, and may become robust after the imaginal molt.
| Original language | English |
|---|---|
| Pages (from-to) | 237-243 |
| Number of pages | 7 |
| Journal | Zoological Science |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Circadian clock
- Clock gene
- Cricket
- Nymph
- Peripheral oscillator
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Post-embryonic development of circadian oscillations within and outside the optic lobe in the cricket, gryllus bimaculatus. / Uryu, Outa; Tomioka, Kenji.
In: Zoological Science, Vol. 31, No. 4, 2014, p. 237-243.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Post-embryonic development of circadian oscillations within and outside the optic lobe in the cricket, gryllus bimaculatus
AU - Uryu, Outa
AU - Tomioka, Kenji
PY - 2014
Y1 - 2014
N2 - The adult cricket Gryllus bimaculatus has a central clock in the optic lobe that regulates overt activity rhythms and secondary oscillators in the tissue outside the optic lobe. Here we investigated properties of the rhythmic expression of clock genes in the optic lobe and extra-optic lobe tissues in nymphs, and compared them with those of adults. In the optic lobe, mRNA of the clock genes period (per), timeless (tim), cycle (cyc) and Clock (Clk) were expressed in patterns similar to those in adult profiles, but at significantly lower levels. Among the extra-optic lobe tissues, the brain and TAG showed a rhythmic expression of per and tim, the mid-gut only in tim, and the anterior-stomach in none of the genes studied. The mRNA levels of clock genes were again significantly lower than those in adults. Unlike in adults, the brain and mid-gut lost their rhythms of clock gene expression in DD, and when the optic lobes were bilaterally removed. These results suggest that the rhythms outside the optic lobe are weak in nymphs, and may become robust after the imaginal molt.
AB - The adult cricket Gryllus bimaculatus has a central clock in the optic lobe that regulates overt activity rhythms and secondary oscillators in the tissue outside the optic lobe. Here we investigated properties of the rhythmic expression of clock genes in the optic lobe and extra-optic lobe tissues in nymphs, and compared them with those of adults. In the optic lobe, mRNA of the clock genes period (per), timeless (tim), cycle (cyc) and Clock (Clk) were expressed in patterns similar to those in adult profiles, but at significantly lower levels. Among the extra-optic lobe tissues, the brain and TAG showed a rhythmic expression of per and tim, the mid-gut only in tim, and the anterior-stomach in none of the genes studied. The mRNA levels of clock genes were again significantly lower than those in adults. Unlike in adults, the brain and mid-gut lost their rhythms of clock gene expression in DD, and when the optic lobes were bilaterally removed. These results suggest that the rhythms outside the optic lobe are weak in nymphs, and may become robust after the imaginal molt.
KW - Circadian clock
KW - Clock gene
KW - Cricket
KW - Nymph
KW - Peripheral oscillator
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UR - http://www.scopus.com/inward/citedby.url?scp=84898439070&partnerID=8YFLogxK
U2 - 10.2108/zs130230
DO - 10.2108/zs130230
M3 - Article
C2 - 24694226
AN - SCOPUS:84898439070
VL - 31
SP - 237
EP - 243
JO - Zoological Science
JF - Zoological Science
SN - 0289-0003
IS - 4
ER -