Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE

Xiao Zhu, Shaohai Wang, Yihai Gu, Xiaoyu Li, Hui Yan, He Yan, Shin-ichi Miyoshi, Lei Shi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although it is well-known that variations of the microbial community in a specific location of human body may be associated with some diseases, the developing change of the oral microbiota related to oral diseases before and after wearing the removable partial dentures (RPD) is not completely understood. In this study, three kinds of samples (saliva, supra-and subgingival plaque, and oral mucosal surfaces) were collected from the 10-patients group at three different times: before, 1-month and 6-months after the treatment. Ten healthy adults were also selected as the control group. Denaturing gradient gel electrophoresis was applied to identify the bacterial profiles and to analyze the dynamics of the oral microbial population in the pre-and post-therapy. The ANOVA of Repeated Measurement Data indicated that, in the saliva and mucosal surfaces, wearing RPDs caused significant change of numbers of amplicons. As many as 607 amplicons were chosen to cut out and re-amplify by PCR. After cloning and sequencing, a total of 16 bacterial genera were identified. The health-associated genera such as Streptococcus, Neisseria, Rothia, Corynebacterium, Leptotrichia, Gemella, Veillonella, Selenomona and Actinomyces tended to decrease, whereas the disease-associated species including Streptococcus mutans tended to increase. In general, wearing RPDs influenced the diversity of the bacterial species in the oral microbial ecosystem. It is noteworthy that the oral environment will be changed from the healthy status towards the disease status after the treatment.

Original languageEnglish
Pages (from-to)2229-2236
Number of pages8
JournalWorld Journal of Microbiology and Biotechnology
Volume28
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Removable Partial Denture
Saliva
Leptotrichia
Gemella
Mouth Diseases
Veillonella
Neisseria
Actinomyces
Corynebacterium
Denaturing Gradient Gel Electrophoresis
Streptococcus mutans
Microbiota
Streptococcus
Human Body
Ecosystem
Organism Cloning
Analysis of Variance
Therapeutics
Polymerase Chain Reaction
Control Groups

Keywords

  • DGGE
  • Pre-and post-therapy
  • Removable partial denture
  • Total oral bacterial community

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE. / Zhu, Xiao; Wang, Shaohai; Gu, Yihai; Li, Xiaoyu; Yan, Hui; Yan, He; Miyoshi, Shin-ichi; Shi, Lei.

In: World Journal of Microbiology and Biotechnology, Vol. 28, No. 5, 05.2012, p. 2229-2236.

Research output: Contribution to journalArticle

Zhu, Xiao ; Wang, Shaohai ; Gu, Yihai ; Li, Xiaoyu ; Yan, Hui ; Yan, He ; Miyoshi, Shin-ichi ; Shi, Lei. / Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE. In: World Journal of Microbiology and Biotechnology. 2012 ; Vol. 28, No. 5. pp. 2229-2236.
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