Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells

Yuko Tatsukawa, Anom Bowolaksono, Ryo Nishimura, Junichi Komiyama, Tomas J. Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Structural luteolysis occurs by apoptosis of luteal cells. The present study examined the effects of activators of well-characterized second messengers on Fas and caspase-3 mRNA expression and on P4 production in luteal cells in order to trace the pro- and anti-apoptotic factors in the bovine corpus luteum (CL). Cultured bovine mid luteal cells were treated for 24 h with a cyclic AMP analogue (8-bromo cyclic AMP; 8br-cAMP; 2.5 mM), a protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate; PMA; 10 μM), or calcium ionophore (A23187; 10 μM). Fas and caspase-3 mRNA expression was inhibited by 8br-cAMP and PMA but was increased by A23187 (P2+ may act as apoptotic factors by stimulating Fas and caspase-3 expression in the bovine luteal cells.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalJournal of Reproduction and Development
Volume52
Issue number4
DOIs
Publication statusPublished - 2006

Fingerprint

Luteal Cells
luteal cells
Adenylate Kinase
protein kinase C
cyclic AMP
Cyclic AMP
Protein Kinase C
caspase-3
Caspase 3
Ions
ions
Calcium
calcium
cattle
Calcimycin
Luteolysis
8-Bromo Cyclic Adenosine Monophosphate
Messenger RNA
luteolysis
Calcium Ionophores

Keywords

  • Anti-apoptotic factor
  • Apoptosis
  • Corpus luteum
  • Cow
  • Second messenger

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells. / Tatsukawa, Yuko; Bowolaksono, Anom; Nishimura, Ryo; Komiyama, Junichi; Acosta, Tomas J.; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 52, No. 4, 2006, p. 517-522.

Research output: Contribution to journalArticle

Tatsukawa, Yuko ; Bowolaksono, Anom ; Nishimura, Ryo ; Komiyama, Junichi ; Acosta, Tomas J. ; Okuda, Kiyoshi. / Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells. In: Journal of Reproduction and Development. 2006 ; Vol. 52, No. 4. pp. 517-522.
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