Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats

Yoshinori Iba, Yukio Sugimoto, Chiaki Kamei, Tohru Masukawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To clarify the role of mucosal mast cells in the lesion sites of colitis induced by dextran sulfate sodium (DSS) in rats, we investigated the histological changes and alterations relevant to mucosal mast cells in the spontaneous recovery process of colitis. Oral administration of 4% DSS solution for 11 days resulted in surface epithelial loss, crypt loss and goblet cell depletion in the rectal mucosa. A marked infiltration of inflammatory cells into the mucosa, which was consistent with a significant increase in myeloperoxidase (MPO) activity, was observed. In addition, mucosal mast cell number and rat mast cell protease (RMCP) I and II levels in the rectum increased at day 0 after DSS treatment, and most of the mucosal mast cells were degranulated. After replacing 4% DSS solution with water, re-epithelialization and restoration of goblet cells were observed at day 5 and day 10, respectively, but crypt damage was hardly recovered even at day 20. The elevated myeloperoxidase activity was significantly decreased from day 5 after DSS treatment. The increased number of mucosal mast cells was further elevated up to about 1.5-fold at day 10 and day 20 after DSS treatment and little degranulation was observed. In the spontaneous recovery process, the increased rat mast cell protease II level in the rectum was maintained for 20 days, while the increased rat mast cell protease I level was gradually decreased and recovered to control level. These results suggest that proliferated mucosal mast cells remained for 20 days, although most of infiltrated inflammatory cells disappeared in spontaneous recovery process of colitis. It may therefore be presumed that proliferated mucosal mast cells play a role in spontaneous recovery process of the colitis induced by DSS.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalInternational Immunopharmacology
Volume3
Issue number4
DOIs
Publication statusPublished - Apr 2003

Fingerprint

Dextran Sulfate
Colitis
Mast Cells
Goblet Cells
Rectum
Peroxidase
Mucous Membrane
Re-Epithelialization
Oral Administration
Peptide Hydrolases
Therapeutics
Cell Count
Water

Keywords

  • Colitis
  • Dextran sulfate sodium
  • Mucosal mast cells
  • Rat mast cell protease
  • Spontaneous recovery process

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats. / Iba, Yoshinori; Sugimoto, Yukio; Kamei, Chiaki; Masukawa, Tohru.

In: International Immunopharmacology, Vol. 3, No. 4, 04.2003, p. 485-491.

Research output: Contribution to journalArticle

@article{cd72dd14f00140e59d892f05c5d51389,
title = "Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats",
abstract = "To clarify the role of mucosal mast cells in the lesion sites of colitis induced by dextran sulfate sodium (DSS) in rats, we investigated the histological changes and alterations relevant to mucosal mast cells in the spontaneous recovery process of colitis. Oral administration of 4{\%} DSS solution for 11 days resulted in surface epithelial loss, crypt loss and goblet cell depletion in the rectal mucosa. A marked infiltration of inflammatory cells into the mucosa, which was consistent with a significant increase in myeloperoxidase (MPO) activity, was observed. In addition, mucosal mast cell number and rat mast cell protease (RMCP) I and II levels in the rectum increased at day 0 after DSS treatment, and most of the mucosal mast cells were degranulated. After replacing 4{\%} DSS solution with water, re-epithelialization and restoration of goblet cells were observed at day 5 and day 10, respectively, but crypt damage was hardly recovered even at day 20. The elevated myeloperoxidase activity was significantly decreased from day 5 after DSS treatment. The increased number of mucosal mast cells was further elevated up to about 1.5-fold at day 10 and day 20 after DSS treatment and little degranulation was observed. In the spontaneous recovery process, the increased rat mast cell protease II level in the rectum was maintained for 20 days, while the increased rat mast cell protease I level was gradually decreased and recovered to control level. These results suggest that proliferated mucosal mast cells remained for 20 days, although most of infiltrated inflammatory cells disappeared in spontaneous recovery process of colitis. It may therefore be presumed that proliferated mucosal mast cells play a role in spontaneous recovery process of the colitis induced by DSS.",
keywords = "Colitis, Dextran sulfate sodium, Mucosal mast cells, Rat mast cell protease, Spontaneous recovery process",
author = "Yoshinori Iba and Yukio Sugimoto and Chiaki Kamei and Tohru Masukawa",
year = "2003",
month = "4",
doi = "10.1016/S1567-5769(02)00299-0",
language = "English",
volume = "3",
pages = "485--491",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats

AU - Iba, Yoshinori

AU - Sugimoto, Yukio

AU - Kamei, Chiaki

AU - Masukawa, Tohru

PY - 2003/4

Y1 - 2003/4

N2 - To clarify the role of mucosal mast cells in the lesion sites of colitis induced by dextran sulfate sodium (DSS) in rats, we investigated the histological changes and alterations relevant to mucosal mast cells in the spontaneous recovery process of colitis. Oral administration of 4% DSS solution for 11 days resulted in surface epithelial loss, crypt loss and goblet cell depletion in the rectal mucosa. A marked infiltration of inflammatory cells into the mucosa, which was consistent with a significant increase in myeloperoxidase (MPO) activity, was observed. In addition, mucosal mast cell number and rat mast cell protease (RMCP) I and II levels in the rectum increased at day 0 after DSS treatment, and most of the mucosal mast cells were degranulated. After replacing 4% DSS solution with water, re-epithelialization and restoration of goblet cells were observed at day 5 and day 10, respectively, but crypt damage was hardly recovered even at day 20. The elevated myeloperoxidase activity was significantly decreased from day 5 after DSS treatment. The increased number of mucosal mast cells was further elevated up to about 1.5-fold at day 10 and day 20 after DSS treatment and little degranulation was observed. In the spontaneous recovery process, the increased rat mast cell protease II level in the rectum was maintained for 20 days, while the increased rat mast cell protease I level was gradually decreased and recovered to control level. These results suggest that proliferated mucosal mast cells remained for 20 days, although most of infiltrated inflammatory cells disappeared in spontaneous recovery process of colitis. It may therefore be presumed that proliferated mucosal mast cells play a role in spontaneous recovery process of the colitis induced by DSS.

AB - To clarify the role of mucosal mast cells in the lesion sites of colitis induced by dextran sulfate sodium (DSS) in rats, we investigated the histological changes and alterations relevant to mucosal mast cells in the spontaneous recovery process of colitis. Oral administration of 4% DSS solution for 11 days resulted in surface epithelial loss, crypt loss and goblet cell depletion in the rectal mucosa. A marked infiltration of inflammatory cells into the mucosa, which was consistent with a significant increase in myeloperoxidase (MPO) activity, was observed. In addition, mucosal mast cell number and rat mast cell protease (RMCP) I and II levels in the rectum increased at day 0 after DSS treatment, and most of the mucosal mast cells were degranulated. After replacing 4% DSS solution with water, re-epithelialization and restoration of goblet cells were observed at day 5 and day 10, respectively, but crypt damage was hardly recovered even at day 20. The elevated myeloperoxidase activity was significantly decreased from day 5 after DSS treatment. The increased number of mucosal mast cells was further elevated up to about 1.5-fold at day 10 and day 20 after DSS treatment and little degranulation was observed. In the spontaneous recovery process, the increased rat mast cell protease II level in the rectum was maintained for 20 days, while the increased rat mast cell protease I level was gradually decreased and recovered to control level. These results suggest that proliferated mucosal mast cells remained for 20 days, although most of infiltrated inflammatory cells disappeared in spontaneous recovery process of colitis. It may therefore be presumed that proliferated mucosal mast cells play a role in spontaneous recovery process of the colitis induced by DSS.

KW - Colitis

KW - Dextran sulfate sodium

KW - Mucosal mast cells

KW - Rat mast cell protease

KW - Spontaneous recovery process

UR - http://www.scopus.com/inward/record.url?scp=0037390592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037390592&partnerID=8YFLogxK

U2 - 10.1016/S1567-5769(02)00299-0

DO - 10.1016/S1567-5769(02)00299-0

M3 - Article

VL - 3

SP - 485

EP - 491

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 4

ER -