Possible role of LRP1, a CCN2 receptor, in chondrocytes

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.

Original languageEnglish
Pages (from-to)552-559
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number2
DOIs
Publication statusPublished - Jun 30 2006

Fingerprint

Cartilage
Chondrocytes
Low Density Lipoprotein Receptor-Related Protein-1
Osteogenesis
LDL Receptors
Recycling
Repair
Endosomes
Lysosomes
Cytoplasm

Keywords

  • Cartilage
  • CCN2/connective tissue growth factor (CTGF)
  • Chondrocyte differentiation
  • Endocytosis
  • Low density lipoprotein receptor-related protein 1 (LRP1)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Possible role of LRP1, a CCN2 receptor, in chondrocytes. / Kawata, Kazumi; Eguchi, Takanori; Kubota, Satoshi; Kawaki, Harumi; Oka, Morihiko; Minagi, Shougo; Takigawa, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 345, No. 2, 30.06.2006, p. 552-559.

Research output: Contribution to journalArticle

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