Abstract
Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.
| Original language | English |
|---|---|
| Pages (from-to) | 552-559 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 345 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 30 2006 |
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Keywords
- Cartilage
- CCN2/connective tissue growth factor (CTGF)
- Chondrocyte differentiation
- Endocytosis
- Low density lipoprotein receptor-related protein 1 (LRP1)
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Possible role of LRP1, a CCN2 receptor, in chondrocytes. / Kawata, Kazumi; Eguchi, Takanori; Kubota, Satoshi; Kawaki, Harumi; Oka, Morihiko; Minagi, Shougo; Takigawa, Masaharu.
In: Biochemical and Biophysical Research Communications, Vol. 345, No. 2, 30.06.2006, p. 552-559.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Possible role of LRP1, a CCN2 receptor, in chondrocytes
AU - Kawata, Kazumi
AU - Eguchi, Takanori
AU - Kubota, Satoshi
AU - Kawaki, Harumi
AU - Oka, Morihiko
AU - Minagi, Shougo
AU - Takigawa, Masaharu
PY - 2006/6/30
Y1 - 2006/6/30
N2 - Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.
AB - Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.
KW - Cartilage
KW - CCN2/connective tissue growth factor (CTGF)
KW - Chondrocyte differentiation
KW - Endocytosis
KW - Low density lipoprotein receptor-related protein 1 (LRP1)
UR - http://www.scopus.com/inward/record.url?scp=33646853310&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646853310&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.04.109
DO - 10.1016/j.bbrc.2006.04.109
M3 - Article
VL - 345
SP - 552
EP - 559
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -