Possible role of LRP1, a CCN2 receptor, in chondrocytes

Kazumi Kawata; Takanori Eguchi; Satoshi Kubota; Harumi Kawaki; Morihiko Oka; Shogo Minagi; Masaharu Takigawa

doi:10.1016/j.bbrc.2006.04.109

Possible role of LRP1, a CCN2 receptor, in chondrocytes

Kazumi Kawata, Takanori Eguchi, Satoshi Kubota, Harumi Kawaki, Morihiko Oka, Shogo Minagi, Masaharu Takigawa

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.

Original language	English
Pages (from-to)	552-559
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	345
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.04.109
Publication status	Published - Jun 30 2006

Keywords

CCN2/connective tissue growth factor (CTGF)
Cartilage
Chondrocyte differentiation
Endocytosis
Low density lipoprotein receptor-related protein 1 (LRP1)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{5bcc285577f94a91aa5bcfe268b9d21d,

title = "Possible role of LRP1, a CCN2 receptor, in chondrocytes",

abstract = "Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.",

keywords = "CCN2/connective tissue growth factor (CTGF), Cartilage, Chondrocyte differentiation, Endocytosis, Low density lipoprotein receptor-related protein 1 (LRP1)",

author = "Kazumi Kawata and Takanori Eguchi and Satoshi Kubota and Harumi Kawaki and Morihiko Oka and Shogo Minagi and Masaharu Takigawa",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research (S) (to M.T.) and (C) (to S.K.) and Exploratory Research (to M.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grants-in-Aid for Fellows of the Japanese Society for the Promotion of Science (to T.E.), and grants from the Sumitomo Foundation (to M.T.), Kurozumi Medical Foundation (to S.K.) and the Nakatomi Foundation (to S.K.).",

year = "2006",

month = jun,

day = "30",

doi = "10.1016/j.bbrc.2006.04.109",

language = "English",

volume = "345",

pages = "552--559",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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T1 - Possible role of LRP1, a CCN2 receptor, in chondrocytes

AU - Kawata, Kazumi

AU - Eguchi, Takanori

AU - Kubota, Satoshi

AU - Kawaki, Harumi

AU - Oka, Morihiko

AU - Minagi, Shogo

AU - Takigawa, Masaharu

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research (S) (to M.T.) and (C) (to S.K.) and Exploratory Research (to M.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grants-in-Aid for Fellows of the Japanese Society for the Promotion of Science (to T.E.), and grants from the Sumitomo Foundation (to M.T.), Kurozumi Medical Foundation (to S.K.) and the Nakatomi Foundation (to S.K.).

PY - 2006/6/30

Y1 - 2006/6/30

N2 - Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.

AB - Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.

KW - CCN2/connective tissue growth factor (CTGF)

KW - Cartilage

KW - Chondrocyte differentiation

KW - Endocytosis

KW - Low density lipoprotein receptor-related protein 1 (LRP1)

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DO - 10.1016/j.bbrc.2006.04.109

M3 - Article

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EP - 559

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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