Possible role of interferon-τ on in vitro development of bovine embryos

Masashi Takahashi, Hitomi Takahashi, Seizo Hamano, Satoko Watanabe, Shigeki Inumaru, Masaya Geshi, Kiyoshi Okuda, Yuichi Yokomizo, Akira Okano

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of interferon-τ on in vitro development of bovine embryos was investigated. After in vitro fertilization, embryos developed to the morula stage were cultured for 3 days in TCM-199 or CR1 medium containing BSA or FCS supplemented with or without recombinant IFN-τ produced by a baculovirus expression system. Addition of baculovirus-expressed IFN-τ (100 ng/ml) significantly promoted development to the blastocyst stage in both culture media. Addition of E. coli expressed IFN-τ (2 μg/ml) also significantly promoted the embryonic development. Supplementation of BSA or FCS did not affect the growth-promoting effect of IFN-τ. To determine whether the growth-promoting effect of IFN-τ is related to the interferon type I receptors that bind to type I interferon such as IFN-α, embryos were cultured with IFN-α. Although IFN-α significantly promoted the development, a much higher concentration (25 μg/ml) was required than IFN-α. A reverse transcription polymerase chain reaction analysis revealed the expression of mRNA encoded type-I IFN receptor subunit from morula to blastocyst stage embryos. The overall results suggest a novel function for IFNs in promoting embryonic development and the effect may be related to type-I IFN receptor expressed in the early stages of preimplantation embryos.

Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalJournal of Reproduction and Development
Volume49
Issue number4
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Bovine embryo
  • Development
  • Interferon type I receptor
  • Interferon-τ

ASJC Scopus subject areas

  • Animal Science and Zoology

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  • Cite this

    Takahashi, M., Takahashi, H., Hamano, S., Watanabe, S., Inumaru, S., Geshi, M., Okuda, K., Yokomizo, Y., & Okano, A. (2003). Possible role of interferon-τ on in vitro development of bovine embryos. Journal of Reproduction and Development, 49(4), 297-305. https://doi.org/10.1262/jrd.49.297