Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica)

Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although a rise in intracellular calcium concentration of vertebrate oocytes plays a pivotal role for the initiation of fertilization or oocyte activation, no study on this subject has been reported in birds. This study was conducted to study the role of intracellular calcium in relation to fertilization in avian oocytes. First, immediately after a quail oocyte was injected with a sperm, it was treated with strontium chloride as an inducer for intracellular calcium rise at doses of 0, 2.5, 5, 7.5, 10 mM for 4 hr in the culture medium and was followed by 20-hr culture. Treatment with 5 mM of strontium chloride induced blastodermal development in 24.2% of injected eggs, although no oocytes developed without strontium treatment. Second, quail oocytes were injected with a sperm and 0.1 M calcium chloride or a sperm and saline solution, cultured without calcium for 4hr and was followed by 20-hr culture without strontium. The calcium solution induced blastodermal development in 20.5% of the oocytes, although no oocytes developed without calcium treatment. Third, quail oocytes were injected with 1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA) as a calcium chelator, cultured with strontium (5 mM) for 4hr followed by 20-hr culture without strontium. Only one oocyte developed after BAPTA and strontium treatment of 36 oocytes examined. Developmental stages of all the oocytes ranged from IV to VII. These results suggest that intracellular calcium rise may participate in quail oocyte activation and allow fertilization and blastodermal development.

Original languageEnglish
Pages (from-to)647-653
Number of pages7
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume307
Issue number11
DOIs
Publication statusPublished - Nov 1 2007
Externally publishedYes

Fingerprint

intracytoplasmic sperm injection
Coturnix
Quail
Intracytoplasmic Sperm Injections
quails
sperm
Oocytes
oocytes
strontium
calcium
Calcium
Strontium
chloride
Fertilization
Spermatozoa
spermatozoa
fertilization (reproduction)
Coturnix japonica
chlorides
ethane

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Physiology
  • Molecular Biology

Cite this

Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica). / Mizushima, Shusei; Takagi, Soichi; Ono, Tamao; Atsumi, Yusuke; Tsukada, Akira; Saito, Noboru; Shimada, Kiyoshi.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 307, No. 11, 01.11.2007, p. 647-653.

Research output: Contribution to journalArticle

Mizushima, Shusei ; Takagi, Soichi ; Ono, Tamao ; Atsumi, Yusuke ; Tsukada, Akira ; Saito, Noboru ; Shimada, Kiyoshi. / Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica). In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2007 ; Vol. 307, No. 11. pp. 647-653.
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