Possible pathway for the processing of sugar chains containing xylose in plant glycoproteins deduced on structural analyses of sugar chains from Ricinus communis lectins

Yoshinobu Kimura, Sumihiro Hase, Yuji Kobayashi, Yoshimasa Kyogoku, Gunki Funatsu, Tokuji Ikenaka

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The structures of sugar chains from two lectins in seeds of the castor bean (Ricinus communis) were identified. The sugar chains were liberated from the lectins by hydrazinolysis. After N-acetylation, the reducing-end residues of the sugar chains were coupled with 2-aminopyridine. The pyridylamino derivatives thus obtained were purified by gel filtration and HPLC. The structures of the purified derivatives were identified by component sugar analysis, stepwise exoglycosidase digestion, partial acetolysis, and 500 MHz 1H-NMR spectroscopy. A new processing pathway for sugar chains in plant glycoproteins was proposed on the basis of the structures of the sugar chains.

Original languageEnglish
Pages (from-to)1051-1054
Number of pages4
JournalJournal of Biochemistry
Volume101
Issue number4
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Ricinus Communis
Lectin
Glycoproteins
Xylose
Glycoprotein
Sugars
Lectins
Pathway
Castor Bean
Ricinus
Glycoside Hydrolases
Acetylation
Processing
Gel Chromatography
Digestion
Seeds
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Derivatives
Derivative

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Possible pathway for the processing of sugar chains containing xylose in plant glycoproteins deduced on structural analyses of sugar chains from Ricinus communis lectins. / Kimura, Yoshinobu; Hase, Sumihiro; Kobayashi, Yuji; Kyogoku, Yoshimasa; Funatsu, Gunki; Ikenaka, Tokuji.

In: Journal of Biochemistry, Vol. 101, No. 4, 1987, p. 1051-1054.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Hase, Sumihiro ; Kobayashi, Yuji ; Kyogoku, Yoshimasa ; Funatsu, Gunki ; Ikenaka, Tokuji. / Possible pathway for the processing of sugar chains containing xylose in plant glycoproteins deduced on structural analyses of sugar chains from Ricinus communis lectins. In: Journal of Biochemistry. 1987 ; Vol. 101, No. 4. pp. 1051-1054.
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