Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli

Bunichi Ezaki, Hirotada Mori, Teru Ogura, Sota Hiraga

Research output: Contribution to journalArticle

Abstract

The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42° C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg- phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30° C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalMGG Molecular & General Genetics
Volume223
Issue number3
DOIs
Publication statusPublished - Sep 1990
Externally publishedYes

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Keywords

  • F plasmid
  • Heat shock response
  • Phosphate transport system
  • Plasmid maintenance
  • seg-3 mutant

ASJC Scopus subject areas

  • Genetics

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