Possible involvement of oxytocin and its receptor in the local regulation of prostaglandin secretion in the cat endometrium

Marta J. Siemieniuch, Jaroslaw J. Mlynarczuk, Dariusz J. Skarzynski, Kiyoshi Okuda

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13 Citations (Scopus)


Ovarian originated oxytocin (OT) is involved in several reproductive process, amongst them its role in the regulation/modulation of the estrous cycle in several species has been demonstrated. Although the systemic role of endometrial originated prostaglandins (PGs), especially prostaglandin F (PGF), is equivocal in cats, their possible involvement in the local regulation of uterine events during the estrous cycle is uncertain. We examined the spontaneous and LH-stimulated OT production in cultured luteal cells, the spatial and temporal arrangement of OT receptors (OTR) in a cat endometrium and, finally the effects of OT on PG secretion and prostaglandin-endoperoxide synthase (PTGS2) expression in the feline cultured endometrial cells. Uteri together with ovaries were collected from adult domestic cats (n=27) at different stages of the estrous cycle, after routine ovariohisterectomy procedures. The endometrial and luteal cells were separated enzymatically. Luteinizing hormone (LH) augmented OT secretion in cultured luteal cells 2-fold compared with control (P<0.05). Oxytocin receptor was abundantly expressed in different ovarian structure, as well as in uterine tissues collected at early/developing and mid-luteal phase. The secretion of PGF by endometrial epithelial cells was increased by OT at a dose 10-7M (P<0.001). Atosiban (specific OTR blocker) alone did not affect PG secretion but atosiban in combination with OT abolished the stimulating effect of OT on PGF secretion. Oxytocin augmented PGE2 secretion at a dose 10-7M and 10-6M in the endometrial stromal cells (P<0.001). The treatment with atosiban did not abrogated positive effect of OT on PGE2 production in the stromal cells. Effect of OT on PTGS2 mRNA expression, the rate-limiting enzyme in PG production, was examined by Real Time-PCR and PTGS2 mRNA expression was significantly affected by OT in both epithelial and stromal cell cultures (P<0.01). The present observations have shown that OT is locally produced by the early/developing corpora lutea and that corpora lutea delivered OT may regulate PG secretion in a cat endometrium especially at early- and mid-diestrus, by affecting PTGS2 mRNA expression.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalAnimal Reproduction Science
Issue number1-2
Publication statusPublished - Jan 1 2011


  • Cat
  • Corpus luteum
  • Endometrium
  • Oxytocin
  • Prostaglandin

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology


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