Possible involvement of oxytocin and its receptor in the local regulation of prostaglandin secretion in the cat endometrium

Marta J. Siemieniuch, Jaroslaw J. Mlynarczuk, Dariusz J. Skarzynski, Kiyoshi Okuda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ovarian originated oxytocin (OT) is involved in several reproductive process, amongst them its role in the regulation/modulation of the estrous cycle in several species has been demonstrated. Although the systemic role of endometrial originated prostaglandins (PGs), especially prostaglandin F (PGF), is equivocal in cats, their possible involvement in the local regulation of uterine events during the estrous cycle is uncertain. We examined the spontaneous and LH-stimulated OT production in cultured luteal cells, the spatial and temporal arrangement of OT receptors (OTR) in a cat endometrium and, finally the effects of OT on PG secretion and prostaglandin-endoperoxide synthase (PTGS2) expression in the feline cultured endometrial cells. Uteri together with ovaries were collected from adult domestic cats (n=27) at different stages of the estrous cycle, after routine ovariohisterectomy procedures. The endometrial and luteal cells were separated enzymatically. Luteinizing hormone (LH) augmented OT secretion in cultured luteal cells 2-fold compared with control (P2α by endometrial epithelial cells was increased by OT at a dose 10-7M (P2α secretion. Oxytocin augmented PGE2 secretion at a dose 10-7M and 10-6M in the endometrial stromal cells (P2 production in the stromal cells. Effect of OT on PTGS2 mRNA expression, the rate-limiting enzyme in PG production, was examined by Real Time-PCR and PTGS2 mRNA expression was significantly affected by OT in both epithelial and stromal cell cultures (P

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalAnimal Reproduction Science
Volume123
Issue number1-2
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Oxytocin Receptors
endometrium
oxytocin
Oxytocin
Endometrium
prostaglandins
Prostaglandins
Cats
secretion
cats
receptors
Luteal Cells
luteal cells
Estrous Cycle
stromal cells
Cyclooxygenase 2
Stromal Cells
estrous cycle
cultured cells
Cultured Cells

Keywords

  • Cat
  • Corpus luteum
  • Endometrium
  • Oxytocin
  • Prostaglandin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Endocrinology

Cite this

Possible involvement of oxytocin and its receptor in the local regulation of prostaglandin secretion in the cat endometrium. / Siemieniuch, Marta J.; Mlynarczuk, Jaroslaw J.; Skarzynski, Dariusz J.; Okuda, Kiyoshi.

In: Animal Reproduction Science, Vol. 123, No. 1-2, 01.2011, p. 89-97.

Research output: Contribution to journalArticle

Siemieniuch, Marta J. ; Mlynarczuk, Jaroslaw J. ; Skarzynski, Dariusz J. ; Okuda, Kiyoshi. / Possible involvement of oxytocin and its receptor in the local regulation of prostaglandin secretion in the cat endometrium. In: Animal Reproduction Science. 2011 ; Vol. 123, No. 1-2. pp. 89-97.
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