Abstract
The Ex-tended black (E) locus on chromosome 1 acts within the melanocyte to regulate feather color pigmentation in the chicken. Several alleles exhibiting different pigmentation have been described and their phenotypes are similar to those of the murine extension locus which encodes melanocortin 1-receptor (MC1-R), the receptor for α-melanocyte-stimulating hormone (α-MSH). To investigate whether the MC1-R gene is responsible for E-locus function, we examined the structure of MC1-R in E-locus mutants by RFLP analysis and genomic DNA sequencing. In the most recessive allele (e(y)), which exhibits a uniformly red-yellow pigmentation, MC1-R was found to contain amino acid substitutions possibly causing functional deficiency. On the other hand, in the most dominant allele (E), which confers a uniformly black pigmentation, MC1-R possessed mutation responsible for a constitutively active MC1-R and resultant black coat color in mice. Our finding that the-structure of MC1-R was affected by individual E-locus alleles strongly suggests that MC1-R is associated with the E-locus. Furthermore, since the same mutation of MC1-R was found in mice and chickens that exhibit the same pigmentation, it is possible that the regulatory mechanism of MC1-R function is shared in chickens and mammals.
| Original language | English |
|---|---|
| Pages (from-to) | 164-168 |
| Number of pages | 5 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1308 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 14 1996 |
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Keywords
- Chicken
- Extended black locus
- Melanocortin 1-receptor
- Pigmentation
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Structural Biology
- Biophysics
Cite this
Possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken. / Takeuchi, Sakae; Suzuki, Hideyuki; Yabuuchi, Masafumi; Takahashi, Sumio.
In: BBA - Gene Structure and Expression, Vol. 1308, No. 2, 14.08.1996, p. 164-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken
AU - Takeuchi, Sakae
AU - Suzuki, Hideyuki
AU - Yabuuchi, Masafumi
AU - Takahashi, Sumio
PY - 1996/8/14
Y1 - 1996/8/14
N2 - The Ex-tended black (E) locus on chromosome 1 acts within the melanocyte to regulate feather color pigmentation in the chicken. Several alleles exhibiting different pigmentation have been described and their phenotypes are similar to those of the murine extension locus which encodes melanocortin 1-receptor (MC1-R), the receptor for α-melanocyte-stimulating hormone (α-MSH). To investigate whether the MC1-R gene is responsible for E-locus function, we examined the structure of MC1-R in E-locus mutants by RFLP analysis and genomic DNA sequencing. In the most recessive allele (e(y)), which exhibits a uniformly red-yellow pigmentation, MC1-R was found to contain amino acid substitutions possibly causing functional deficiency. On the other hand, in the most dominant allele (E), which confers a uniformly black pigmentation, MC1-R possessed mutation responsible for a constitutively active MC1-R and resultant black coat color in mice. Our finding that the-structure of MC1-R was affected by individual E-locus alleles strongly suggests that MC1-R is associated with the E-locus. Furthermore, since the same mutation of MC1-R was found in mice and chickens that exhibit the same pigmentation, it is possible that the regulatory mechanism of MC1-R function is shared in chickens and mammals.
AB - The Ex-tended black (E) locus on chromosome 1 acts within the melanocyte to regulate feather color pigmentation in the chicken. Several alleles exhibiting different pigmentation have been described and their phenotypes are similar to those of the murine extension locus which encodes melanocortin 1-receptor (MC1-R), the receptor for α-melanocyte-stimulating hormone (α-MSH). To investigate whether the MC1-R gene is responsible for E-locus function, we examined the structure of MC1-R in E-locus mutants by RFLP analysis and genomic DNA sequencing. In the most recessive allele (e(y)), which exhibits a uniformly red-yellow pigmentation, MC1-R was found to contain amino acid substitutions possibly causing functional deficiency. On the other hand, in the most dominant allele (E), which confers a uniformly black pigmentation, MC1-R possessed mutation responsible for a constitutively active MC1-R and resultant black coat color in mice. Our finding that the-structure of MC1-R was affected by individual E-locus alleles strongly suggests that MC1-R is associated with the E-locus. Furthermore, since the same mutation of MC1-R was found in mice and chickens that exhibit the same pigmentation, it is possible that the regulatory mechanism of MC1-R function is shared in chickens and mammals.
KW - Chicken
KW - Extended black locus
KW - Melanocortin 1-receptor
KW - Pigmentation
UR - http://www.scopus.com/inward/record.url?scp=0030583297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030583297&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(96)00100-5
DO - 10.1016/0167-4781(96)00100-5
M3 - Article
VL - 1308
SP - 164
EP - 168
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -