Possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken

Sakae Takeuchi, Hideyuki Suzuki, Masafumi Yabuuchi, Sumio Takahashi

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The Ex-tended black (E) locus on chromosome 1 acts within the melanocyte to regulate feather color pigmentation in the chicken. Several alleles exhibiting different pigmentation have been described and their phenotypes are similar to those of the murine extension locus which encodes melanocortin 1-receptor (MC1-R), the receptor for α-melanocyte-stimulating hormone (α-MSH). To investigate whether the MC1-R gene is responsible for E-locus function, we examined the structure of MC1-R in E-locus mutants by RFLP analysis and genomic DNA sequencing. In the most recessive allele (e(y)), which exhibits a uniformly red-yellow pigmentation, MC1-R was found to contain amino acid substitutions possibly causing functional deficiency. On the other hand, in the most dominant allele (E), which confers a uniformly black pigmentation, MC1-R possessed mutation responsible for a constitutively active MC1-R and resultant black coat color in mice. Our finding that the-structure of MC1-R was affected by individual E-locus alleles strongly suggests that MC1-R is associated with the E-locus. Furthermore, since the same mutation of MC1-R was found in mice and chickens that exhibit the same pigmentation, it is possible that the regulatory mechanism of MC1-R function is shared in chickens and mammals.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1308
Issue number2
DOIs
Publication statusPublished - Aug 14 1996

Fingerprint

Receptor, Melanocortin, Type 1
Feathers
Pigmentation
Chickens
Color
Alleles
Mutation
Mammals
Chromosomes, Human, Pair 1
Melanocytes
Amino Acid Substitution
Chromosomes
DNA Sequence Analysis
Restriction Fragment Length Polymorphisms
Substitution reactions
Genes

Keywords

  • Chicken
  • Extended black locus
  • Melanocortin 1-receptor
  • Pigmentation

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken. / Takeuchi, Sakae; Suzuki, Hideyuki; Yabuuchi, Masafumi; Takahashi, Sumio.

In: BBA - Gene Structure and Expression, Vol. 1308, No. 2, 14.08.1996, p. 164-168.

Research output: Contribution to journalArticle

Takeuchi, Sakae ; Suzuki, Hideyuki ; Yabuuchi, Masafumi ; Takahashi, Sumio. / Possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken. In: BBA - Gene Structure and Expression. 1996 ; Vol. 1308, No. 2. pp. 164-168.
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